NDLI: Fabrication and Characterization of a Microscale Cellular Loading Device for Cellular Biomechanical Study

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Wang, Qian Kim, Bongsu Zhang, Xu Zhao, Yi
Copyright Year	2011
Abstract	Mechanical stimuli interfere with cellular behaviors under many physiological conditions. To understand the role of mechanical stimuli, engineered devices are developed to apply mechanical loads to cells in vitro. Despite of their usefulness, these devices are limited since they often lack the capacity of spatial load control, which is essential for intercellular study. Moreover, application of both compressive and tensile loads using a single loading device is challenging. Here, we fabricate and characterize a microdevice for applying programmable compressive/tensile loads to live cells. The device consists of two PDMS substrates. The top substrate consists of nine circular membranes with patterned microdots array on the top surfaces. Each membrane is connected with a microfluidic channel built in the bottom substrate. Upon actuation, the fluid in the channels deforms the membranes and applies controllable strain to cells cultured on the membranes. In this design, each membrane can be individually controlled to apply desired strain levels. The surface strain of the PDMS membranes is characterized by mapping the displacement of the dot array. The result of strain analysis shows that, the radial strain at the center of a circular membrane upon deformation ranges from about 5% compressive strain to about 20% tensile strain, validating the capacity of the device in applying both tensile and compressive stresses. Cell testing is performed using trabecular meshwork endothelial cells. Cells on different membranes are subjected to 0.5Hz of compressive or tensile stresses. The result shows that compressive and tensile stresses have different effects on the cells, indicating the device a promising solution for cellular biomechanical study.
Starting Page	949
Ending Page	953
Page Count	5
File Format	PDF
ISBN	9780791854884
DOI	10.1115/IMECE2011-65188
Volume Number	Volume 2: Biomedical and Biotechnology Engineering; Nanoengineering for Medicine and Biology
Conference Proceedings	ASME 2011 International Mechanical Engineering Congress and Exposition
Language	English
Publisher Date	2011-11-11
Publisher Place	Denver, Colorado, USA
Access Restriction	Subscribed
Subject Keyword	Membranes Deformation Microscale devices Plasma desorption mass spectrometry Stress Displacement Endothelial cells Biomechanics Design Compressive stress Fluids Tension Physiology Manufacturing Microfluidics Testing
Content Type	Text
Resource Type	Article

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