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Improved Method for Analysis of Airborne Asbestos Fibers Using Phase Contrast Microscopy and FTIR Spectrometry
| Content Provider | Semantic Scholar |
|---|---|
| Author | Azari, Mansour Rezazadeh Yazdian, A. Zendehdel, Rezvan Souri, Hamid Khodakarim, Soheila Peirovi, Habibalalah Panahi, Davod Kazempour, Marzieh |
| Copyright Year | 2014 |
| Abstract | BACKGROUND In recent years, some studies have tried to improve Phase Contrast Microscopy (PCM) for counting asbestos fibers. Due to the lack of a universally accepted alternative method, this study aimed to improve PCM for better counting of asbestos fibers. MATERIALS AND METHODS Confirmed asbestos standards were applied using a dust generator for sampling. Sampling from the dust generator was carried out according to the Occupational Safety and Health Administration (OSHA) ID-160 method and 95 samples with diverse densities were prepared to be counted using conventional and modern PCM. All samples were counted single blindly by a co-author of this study and the obtained data were analyzed by paired t-test, correlation coefficient and Bland-Altman analysis. Duplicate samples were prepared for qualitative analysis by Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and X ray. RESULTS Asbestos densities on filters were in the range of less than 100 to 600 fibers/mm(2). Statistically, significant differences were observed for the count density of the 95 samples counted by the two phase contrast microscopes (P<0.001). Nikon microscope demonstrated higher counts compared to conventional microscope and had a lower coefficient of variation. Samples were analyzed qualitatively using FT-IR and SEM, and the presence of asbestos fibers was confirmed. CONCLUSION The improved PCM and FT-IR methods presented in this study demonstrated more precise and accurate determination of personal exposure to airborne asbestos fibers and subsequent risk assessment. |
| Starting Page | 38 |
| Ending Page | 45 |
| Page Count | 8 |
| File Format | PDF HTM / HTML |
| PubMed reference number | 25713590 |
| Journal | Medline |
| Volume Number | 13 |
| Alternate Webpage(s) | http://tanaffosjournal.ir/files_site/paperlist/r_670_150207132525.pdf |
| Alternate Webpage(s) | http://ftp.ncbi.nlm.nih.gov/pub/pmc/d0/15/Tanaffos-13-038.PMC4338051.pdf |
| Journal | Tanaffos |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
