NDLI: DNase I aggravates islet ß-cell apoptosis in type 2 diabetes.

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DNase I aggravates islet ß-cell apoptosis in type 2 diabetes.

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DNase I aggravates islet ß-cell apoptosis in type 2 diabetes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Fu, Yu-Ke Zhao, Zhi-Gang Liu, Teng Zhang, Yue-Ying Li, Ping Zheng, Yan-Fei Zhu, Bin Wang, Lei Li, Xin-Gang Zhang, Lei
Description	Author Affiliation: Zhu B ( Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China.); Zhang L ( Department of Pharmacy, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, P.R. China.); Zhang YY ( Department of Chinese Medicine, Beijing Hepingli Hospital, Beijing 100013, P.R. China.); Wang L ( Department of Chinese Medicine, The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100050, P.R. China.); Li XG ( Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China.); Liu T ( Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China.); Fu YK ( Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China.); Zheng YF ( Department of Chinese Medicine, The Third Affiliated Hospital of Beijing University of Chinese Medicine, Beijing 100050, P.R. China.); Li P ( Department of Nephrology, ChinaJapan Friendship Hospital, Beijing 100029, P.R. China.); Zhao ZG ( Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China.)
Abstract	Deoxyribonuclease I (DNase I) is an endonuclease responsible for the destruction of chromatin during apoptosis. However, its role in diabetes remains unclear. The aim of the current study was to investigate the role of DNase I combined with high glucose levels in ßcell apoptosis. Human samples were collected and the DNase I activity was examined. High glucosecultured INS1 cells were transfected with DNase I small interfering RNA (siRNA) and the cell apoptosis was examined by western blotting and flow cytometry. Cell viability was analyzed by the Cell Counting Kit8 assay. Cell apoptosis resulting from 50 mU/µl DNase I was also observed by flow cytometry, terminal deoxynucleotidyl transferase dUTP nickend labeling stain and western blotting. Compared with healthy controls, the serum DNase I activity of patients with diabetes was significantly increased (P<0.05). In addition, DNase I expression was observed to be significantly increased in human pancreatic tissues. The addition of high glucose upregulated the cell apoptotic rate, whereas DNase I knockdown significantly reduced apoptosis in cells treated with high glucose. In addition, the western blotting results indicated that caspase3 was increased subsequent to treatment of cells with 30 mM high glucose, however, this increase can be reversed by transfection with DNase I siRNA (P<0.05). Compared with cells cultured in normal conditions and high glucose, 50 mU/µl DNase I was able to significantly increase the cell apoptotic rate and level of caspase-3. DNase I activity was observed to be increased in type 2 diabetes, and high glucose combined with increased DNase I is suggested to aggravate ß-cell apoptosis.
ISSN	17912997
e-ISSN	17913004
DOI	10.3892/mmr.2016.5102
Journal	Molecular Medicine Reports
Issue Number	6
Volume Number	13
Language	English
Publisher	Spandidos Publications
Publisher Date	2016-06-01
Publisher Place	Greece
Access Restriction	Open
Subject Keyword	Discipline Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology

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