NDLI: Lack of correlation between the spatial distribution of A2E and lipofuscin fluorescence in the human retinal pigment epithelium.

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Lack of correlation between the spatial distribution of A2E and lipofuscin fluorescence in the human retinal pigment epithelium.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Gutierrez, Danielle Higbee, Daniel Schey, Kevin L. Dahrouj, Mohammad Anderson, David M. Koutalos, Yiannis Ablonczy, Zsolt Crouch, Rosalie K. Grey, Angus C. Hanneken, Anne
Description	Country affiliation: United States Author Affiliation: Ablonczy Z ( Department of Ophthalmology, Medical University of South Carolina, Charleston, South Carolina 29425, USA. ablonczy@musc.edu)
Abstract	PURPOSE: The accumulation of lipofuscin in the RPE is a hallmark of aging in the eye. The best characterized component of lipofuscin is A2E, a bis-retinoid byproduct of the normal retinoid visual cycle, which exhibits a broad spectrum of cytotoxic effects in vitro. The purpose of our study was to correlate the distribution of lipofuscin and A2E across the human RPE. METHODS: Lipofuscin fluorescence was imaged in flat-mounted RPE from human donors of various ages. The spatial distributions of A2E and its oxides were determined using matrix-assisted laser desorption-ionization imaging mass spectrometry (MALDI-IMS) on flat-mounted RPE tissue sections and retinal cross-sections. RESULTS: Our data support the clinical observations of strong RPE fluorescence, increasing with age, in the central area of the RPE. However, there was no correlation between the distribution of A2E and lipofuscin, as the levels of A2E were highest in the far periphery and decreased toward the central region. High-resolution MALDI-IMS of retinal cross-sections confirmed the A2E localization data obtained in RPE flat-mounts. Singly- and doubly-oxidized A2E had distributions similar to A2E, but represented <10% of the A2E levels. CONCLUSIONS: This report to our knowledge is the first description of the spatial distribution of A2E in the human RPE by imaging mass spectrometry. These data demonstrate that the accumulation of A2E is not responsible for the increase in lipofuscin fluorescence observed in the central RPE with aging.
ISSN	01460404
e-ISSN	15525783
DOI	10.1167/iovs.13-12250
Journal	Investigative Opthalmology & Visual Science
Issue Number	8
Volume Number	54
Language	English
Publisher	Association for Research in Vision and Ophthalmology
Publisher Date	2013-08-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Aging Metabolism Lipofuscin Biosynthesis Retinal Degeneration Retinal Pigment Epithelium Retinoids Adolescent Biological Markers Child, Preschool Infant Infant, Newborn Pyridinium Compounds Pathology Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Comparative Study Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Discipline Ophthalmology
Content Type	Text
Resource Type	Article
Subject	Ophthalmology Sensory Systems Cellular and Molecular Neuroscience

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