NDLI: A prototype hyperspectral system with a tunable laser source for retinal vessel imaging.

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A prototype hyperspectral system with a tunable laser source for retinal vessel imaging.

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Selection of Phototransduction Genes in Homo sapiens.

IGFBP-3 and TNF- regulate retinal endothelial cell apoptosis.

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Development of latanoprost-loaded biodegradable nanosheet as a new drug delivery system for glaucoma.

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OCT predicts VEGF levels in human eyes.

AMD-associated variants at the chromosome 10q26 locus and the stability of ARMS2 transcripts.

Rethinking A2E.

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Passing-Bablok regression is inappropriate for assessing association between structure and function in glaucoma.

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A prototype hyperspectral system with a tunable laser source for retinal vessel imaging.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sylvestre, Jean-Philippe Hudson, Chris Shahidi, Ayda M. Patel, Sunni R. Flanagan, John G.
Description	Country affiliation: Canada Author Affiliation: Patel SR ( Department of Ophthalmology and Vision Sciences, University Health Network, Toronto Western Hospital, Toronto, Canada. sunni_patel@hotmail.com)
Abstract	PURPOSE: To describe the technology and determine the within-session repeatability of manual retinal reflectance measurements of arterioles and venules using a prototype hyperspectral retinal camera. METHODS: Six healthy young volunteers (three males, average age 26 ± 4 years) had five repeated sets of retinal images captured between 500 and 600 nm at 5-nm intervals using a newly developed hyperspectral retinal camera. Optical densities were manually extracted for first-degree arterioles and venules and the repeatability of retinal reflectance was compared sequentially. The SDs of the differences between sequential mean values were used as an indication of the variance, while the coefficient of repeatability (COR) and intraclass correlation coefficient (ICC) were used to assess repeatability. RESULTS: The mean difference between each sequential measure was calculated using 21 images from each of the five spectral cubes. The SDs of these values ranged from 0.01 to 0.06 OD units and from 0.01 to 0.07 OD units for first-degree arterioles and venules, respectively. The COR ranged from 0.02 to 0.11 OD units (relative to a mean OD of 0.15 [0.06-0.23] OD units) for arterioles and 0.03 to 0.14 OD units (relative to a mean OD of 0.25 [0.17-0.31] OD units) for venules. Good reliability (P < 0.001) was found for arterioles (ICC: 78.8%-94.4% with a Cronbach's of 89.6%-97.6%) and for venules (ICC: 63.7%-92.1% with a Cronbach's of 86.2%-98.1%). CONCLUSIONS: Manual optical density determination with this novel hyperspectral camera showed very good intrasession (and intraobserver) repeatability with a small degree of variance that should form the basis of reliable retinal oxygen saturation values in future imaging research studies. Future automation of retinal vessel reflectance image analyses will likely further improve this repeatability.
ISSN	01460404
e-ISSN	15525783
Journal	Investigative Opthalmology & Visual Science
Issue Number	8
Volume Number	54
Language	English
Publisher	Association for Research in Vision and Ophthalmology
Publisher Date	2013-08-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Arterioles Cytology Diagnostic Techniques, Ophthalmological Instrumentation Lasers Retinal Vessels Venules Reference Values Reproducibility Of Results Comparative Study Discipline Ophthalmology
Content Type	Text
Resource Type	Article
Subject	Ophthalmology Sensory Systems Cellular and Molecular Neuroscience

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