| Author |
Kuijer, Roel Renardel de Lavalette, Victor W. Van Der Worp, Roelofje J. Li, Xiao-Rong Bu, Shao-Chong Postma, Gina Hooymans, Johanna M. M. Los, Leonoor I. |
| Description |
Author Affiliation: Bu SC ( Department of Ophthalmology (BB61), University of Groningen, University Medical Center Groningen, RB Groningen, The Netherlands 2W.J. Kolff Institute, Graduate School of Medical Sciences, University of Groningen, Groningen, The Netherlands 3Tianjin Medica.); Kuijer R ( W.J. Kolff Institute, Graduate School of Medical Sciences, University of Groningen, Groningen, The Netherlands 4Department of Biomedical Engineering (FB40), University of Groningen, University Medical Center Groningen, AV Groningen, The Netherlands.); van der Worp RJ ( Department of Ophthalmology (BB61), University of Groningen, University Medical Center Groningen, RB Groningen, The Netherlands 2W.J. Kolff Institute, Graduate School of Medical Sciences, University of Groningen, Groningen, The Netherlands.); Postma G ( Department of Ophthalmology (BB61), University of Groningen, University Medical Center Groningen, RB Groningen, The Netherlands.); Renardel de Lavalette VW ( Department of Ophthalmology (BB61), University of Groningen, University Medical Center Groningen, RB Groningen, The Netherlands.); Li XR ( Tianjin Medical University Eye Centre, Nan Kai District, Tianjin, China.); Hooymans JM ( Department of Ophthalmology (BB61), University of Groningen, University Medical Center Groningen, RB Groningen, The Netherlands 2W.J. Kolff Institute, Graduate School of Medical Sciences, University of Groningen, Groningen, The Netherlands.); Los LI ( Department of Ophthalmology (BB61), University of Groningen, University Medical Center Groningen, RB Groningen, The Netherlands 2W.J. Kolff Institute, Graduate School of Medical Sciences, University of Groningen, Groningen, The Netherlands.) |
| Abstract |
PURPOSE: The purpose of this study was to investigate the presence of type VI collagen and glial cells in idiopathic epiretinal membrane (iERM) and the role of TGF-ß in the expression of collagens and -smooth muscle actin ( -SMA) in retinal Müller cells. METHODS: Idiopathic ERM samples from vitrectomy were analyzed for glial acidic fibrillary protein (GFAP), cellular retinaldehyde-binding protein (CRALBP), -SMA, and type VI collagen using flat-mount immunohistochemistry. To study intracellular collagen expression in relation to cellular phenotype, spontaneously immortalized human Müller cells (MIO-M1) were treated with TGF-ß1 for 48 hours, and the expression of -SMA and intracellular type I, II, IV, and VI collagens was studied by using immunocytology. Findings in Müller cells were compared with those in fetal lung fibroblasts and newborn skin fibroblasts. RESULTS: A colocalization of GFAP/CRALBP and GFAP/ -SMA was found in iERM, indicating a dynamic process of activation of retinal Müller cells in vivo. Transforming growth factor-ß1 induced up-regulation of -SMA stress fibers in retinal Müller cells and both types of fibroblasts in vitro. The intracellular staining intensity of type I, II, and VI collagens was decreased in retinal Müller cells containing -SMA stress fibers, whereas the intracellular staining intensity of type I and VI collagens in both types of fibroblasts was not affected. CONCLUSIONS: Type VI collagen and activated retinal Müller cells are present in iERM. Transforming growth factor-ß1 induces an up-regulation of -SMA stress fibers in retinal Müller cells and fibroblasts and appears to have a cell-specific effect on intracellular collagen expression. |
