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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wang, X. Y. Li, Q. Dong, W. H. Zhao, C. P. Zhang, J. H. Wang, F. Yang, X. J. Wang, T. Y. Yang, R. Wang, L. |
| Description | Country affiliation: China Author Affiliation: Wang F ( Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.); Zhang JH ( Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.); Wang TY ( Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China wtianyuncn@126.com.); Dong WH ( Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.); Yang XJ ( Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.); Wang XY ( Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.); Wang L ( Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.); Yang R ( Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.); Li Q ( Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.); Zhao CP ( Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan, China.) |
| Abstract | We investigated the effects of different directions of insertion of matrix attachment region (MAR) sequences on transgenic expression in stably transformed Chinese hamster ovary (CHO) cells. The MAR sequences were inserted in forward or reverse directions into the expression vectors, and transfected into CHO cells. The expression of the chloramphenicol acetyltransferase (CAT) reporter gene and the relative copy numbers of the CAT gene were analyzed. The CAT gene expression levels in the vector with the MAR sequence inserted in the forward or reverse directions increased compared with expression without the MAR sequence. The relative copy numbers of the CAT gene with MAR sequenced vectors inserted in the reverse and forward directions were lower, than in the control group. The direction of insertion of MAR sequences had no significant effect on expression levels. The expression levels were not proportional to the copy numbers of the gene. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 2 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-06-26 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Chloramphenicol O-acetyltransferase Genetics Dna, Intergenic Genetic Vectors Chemistry Matrix Attachment Regions Plasmids Animals Cho Cells Cell Line, Transformed Metabolism Cricetulus Gene Dosage Gene Expression Regulation Genes, Reporter Transgenes Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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