| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Zheng, W. H. Liu, W. Xing, R. C. Zheng, J. Yao, R. C. Qin, Z. P. |
| Description |
Country affiliation: China Author Affiliation: Xing RC ( Institute of Hepatopancreatobiliary Surgery,China Three Gorges University, Yichang, Hubei, China.); Zheng J ( Institute of Hepatopancreatobiliary Surgery,China Three Gorges University, Yichang, Hubei, China zhengjun1995@163.com.); Zheng WH ( Department of Pharmacology, Medical Science College, China Three Gorges University, Yichang Hubei, China.); Qin ZP ( Department of Cardiothoracic Surgery, People's Hospital of China Three Gorges University, Yichang, Hubei, China.); Liu W ( Institute of Hepatopancreatobiliary Surgery,China Three Gorges University, Yichang, Hubei, China.); Yao RC ( Institute of Hepatopancreatobiliary Surgery,China Three Gorges University, Yichang, Hubei, China.) |
| Abstract |
We examined the effect of E-cadherin expression on epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) molecular targeted therapy sensitivity/resistance. We treated MCF-7, MDA-MB-231, T24, SiHa, H460, SK-HEP-1, MHCC97-H, and THP-1 cells with the EGFR-TKIs PD153035 and gefitinib, and then tested the drug-resistance and sensitivity using the MTT method, calculated IC50 values for each cell line, and compared the results to E-cadherin content. The MTT assay was used to determine the survival rates of MCF-7, MDA-MB-231, T24, SiHa, H460, SK-HEP-1, MHCC97-H, and THP-1 cells upon the action of EGFR-TKI (PD153035, gefitinib). For PD153035, the IC50 in MCF-7, MDA-MB-231, T24, and SiHa cells differed from that of H460, SK-HEP-1, MHCC97-H, and THP-1 (P < 0.05). Following gefitinib treatment, the IC50 values of MCF-7, MDA-MB-231, T24, and SiHa cells differed from those of H460, SK-HEP-1, MHCC97-H, and THP-1 cells (P < 0.01). The survival rate of MCF-7, MDA-MB-231, T24, and SiHa cells clearly decreased with increasing drug concentration, indicating the cells were sensitive to the drugs and that E-cadherin expression was positive; however, H460, SK-HEP-1, MHCC97-H, and THP-1 cells showed no significant decreased with increasing drug concentration, indicating that they were resistant to the drugs and that E-cadherin expression was negative. The survival rate of epithelial tumor cells through the action of EGFR-TKI is related to E-cadherin expression. E-cadherin may play a significant role in the sensitivity regulation of EGFR molecular targeting treatment. E-cadherin may provide important clues for selecting proper EGFR-TKI molecular targeting treatment. |
| e-ISSN |
16765680 |
| Journal |
Genetics and Molecular Research |
| Issue Number |
2 |
| Volume Number |
14 |
| Language |
English |
| Publisher |
Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date |
2015-05-29 |
| Publisher Place |
Brazil |
| Access Restriction |
Open |
| Subject Keyword |
Breast Neoplasms Drug Therapy Genetics Cadherins Biosynthesis Drug Resistance, Neoplasm Pathology Cell Proliferation Drug Effects Gene Expression Regulation, Neoplastic Mcf-7 Cells Molecular Targeted Therapy Protein Kinase Inhibitors Administration & Dosage Quinazolines Receptor, Epidermal Growth Factor Antagonists & Inhibitors Signal Transduction Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type |
Text |
| Resource Type |
Article |
