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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Hu, M. He, T. Qu, B. H. Wang, D. L. |
| Description | Country affiliation: China Author Affiliation: He T ( Department of Vascular Surgery, The Central Hospital of Wuhan, Wuhan, China HeTao2013086@163.com.); Qu BH ( Department of Vascular Surgery, The Central Hospital of Wuhan, Wuhan, China.); Wang DL ( Department of Vascular Surgery, The Central Hospital of Wuhan, Wuhan, China.); Hu M ( Department of Vascular Surgery, The Central Hospital of Wuhan, Wuhan, China.) |
| Abstract | We investigated the expression and effects of hypoxia-inducible factor-1 (HIF-1 ) in rat thromboangiitis obliterans (TO). Rats were divided into sham and model groups. The model group was further divided into groups based on observation duration. Lauric acid was injected below an artery clamp to simulate TO in the model group; saline was used in the sham group. Clamps were removed 15 min after injection in both groups, and physiological changes were observed at different times (gross observation and hematoxylin and eosin staining). The animals were killed at various times following the operation and serum and muscle tissues were sampled. For the sham group: the endometrium was relatively intact; medial membrane and epineurium lesions were absent; and blood vessels and surrounding tissues had no inflammatory cell infiltration. For the model group: all subgroups displayed inflammation; large numbers of inflammatory cells were gathered; muscle tissue lost its normal texture and structure; and the internal elastic membrane was integrated. Compared with the preoperative status, HIF-1 expression increased significantly in all subgroups (P < 0.05); there was no change in the sham group. HIF-1 expression in each subgroup was different (F = 14.267, P < 0.05). Femoral artery injection of lauric acid can be used as a rat TO model owing to its simple application and success rate. HIF-1 expression increased in the early stage of TO and gradually decreased with the extension of ischemia time; it may play a leading role in TO development and can be used for diagnosis and cure evaluation. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 2 |
| Volume Number | 14 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2015-06-18 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Femoral Artery Pathology Hypoxia-inducible Factor 1, Alpha Subunit Genetics Thromboangiitis Obliterans Animals Disease Models, Animal Disease Progression Eosine Yellowish-(ys) Gene Expression Hematoxylin Histocytochemistry Blood Injections, Intra-arterial Lauric Acids Rats, Sprague-dawley Chemically Induced Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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