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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Iregui, Carlos Vásquez, Gersson Rey, Alba Rivera, Camilo Orozco, Jahir |
| Description | Country affiliation: Colombia Author Affiliation: Vásquez G ( Grupo de Patología Veterinaria, Universidad Nacional de Colombia, Carrera 30 No. 45-03, Bogotá 111321, Colombia.); Rey A ( Grupo de Patología Veterinaria, Universidad Nacional de Colombia, Carrera 30 No. 45-03, Bogotá 111321, Colombia.); Rivera C ( Grupo de Patología Veterinaria, Universidad Nacional de Colombia, Carrera 30 No. 45-03, Bogotá 111321, Colombia.); Iregui C ( Grupo de Patología Veterinaria, Universidad Nacional de Colombia, Carrera 30 No. 45-03, Bogotá 111321, Colombia.); Orozco J ( Max Planck Tandem Group in Nanobioengineering, Universidad de Antioquia, Complejo Ruta N, Calle 67 No. 52-20, Medellín 050010, Colombia. Electronic address: grupotandem@udea.edu.co.) |
| Abstract | Pathogenic bacteria are responsible for several diseases in humans and in a variety of hosts. Detection of pathogenic bacteria is imperative to avoid and/or fight their potential harmful effects. This work reports on the first amperometric biosensor for the rapid detection of Streptococcus agalactiae (S. agalactiae). The biosensor relies on a single biotinylated antibody that immobilizes the bacteria on a screen-printed carbon electrode while is further linked to a streptavidin-conjugated HRP reporter. The biotinylated antibody provides selectivity to the biosensor whereas serves as an anchoring point to the reporter for further amplification of the electrochemical signal. The resultant immunosensor is simple, responds rapidly, and allows for the selective and highly sensitive quantification of S. agalactiae cells in a concentration range of 10 -10 CFUml , with a detection limit of 10CFUml . The approach not only enables a rapid detection and quantification of S. agalactiae in environmental samples but also opens up new opportunities for the simple fabrication of electrochemical immunosensors for different target pathogens. |
| ISSN | 09565663 |
| Volume Number | 87 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-01-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Antibodies, Immobilized Chemistry Biosensing Techniques Methods Fish Diseases Diagnosis Streptococcal Infections Veterinary Streptococcus Agalactiae Isolation & Purification Tilapia Microbiology Water Microbiology Animals Economics Electrochemical Techniques Electrodes Immunoassay Limit Of Detection Journal Article Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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