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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Choi, Jaeyeong Na, Sungsoo Park, Chanho Jang, Kuewhan |
| Description | Author Affiliation: Jang K ( Department of Mechanical Engineering, Korea University, Seoul, 136-701 Republic of Korea.); Choi J ( Department of Mechanical Engineering, Korea University, Seoul, 136-701 Republic of Korea.); Park C ( Department of Mechanical Engineering, Korea University, Seoul, 136-701 Republic of Korea.); Na S ( Department of Mechanical Engineering, Korea University, Seoul, 136-701 Republic of Korea. Electronic address: nass@korea.ac.kr.) |
| Abstract | Assessment of Kirsten rat sarcoma viral oncogene homolog (KRAS) and epidermal growth factor receptor (EGFR) mutations are essential for targeted therapies of patients with non-small-cell lung cancer. In this report, we propose a label-free and high-sensitive detection method of KRAS and EGFR mutations using KPFM and a gold nanoparticle (AuNP)-based platform that densely adsorbs probe DNA and minimizes the sensing area. The detection is based on the evaluation of the surface potential of each AuNP. When AuNPs are modified with probe DNA (AuNP-pDNA), the surface potential is shifted towards the negative potential due to the negatively charged DNA backbone. When AuNP-pDNA further captures target mutant DNA through DNA hybridization, an additional surface potential shift occurs. The platform is able to detect KRAS mutant DNA (13 mer) and EGFR mutant DNA (84 mer) with a limit of detection (LOD) of 3.3pM. Furthermore, the platform is able to detect selectively the KRAS mutant DNA from its wild-type DNA. Our proposed label-free and high-sensitive KPFM method has shown potential glimpses of a personalized medical diagnosis for cancer patients. |
| ISSN | 09565663 |
| Volume Number | 87 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-01-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Dna Mutational Analysis Methods Dna Probes Chemistry Gold Metal Nanoparticles Proto-oncogene Proteins P21(ras) Genetics Receptor, Epidermal Growth Factor Base Sequence Biosensing Techniques Dna Analysis Humans Microscopy, Atomic Force Mutation Journal Article Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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