| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Hsieh, Cheng-Hung Li, Ying Ho, Ja-An Annie Lai, Chi-Wei Chan, Hsin-Yi Tsai, Chang-Feng Chang, Ying-Feng Wu, Li-Chen |
| Description |
Author Affiliation: Li Y ( BioAnalytical Chemistry and Nanobiomedicine Laboratory, Department of Biochemical Science and Technology, National Taiwan University, Taipei, 10617 Taiwan.); Hsieh CH ( Department of Applied Chemistry, National Chi Nan University, Puli, Nantou, 545 Taiwan.); Lai CW ( Department of Applied Chemistry, National Chi Nan University, Puli, Nantou, 545 Taiwan.); Chang YF ( BioAnalytical Chemistry and Nanobiomedicine Laboratory, Department of Biochemical Science and Technology, National Taiwan University, Taipei, 10617 Taiwan.); Chan HY ( Department of Applied Chemistry, National Chi Nan University, Puli, Nantou, 545 Taiwan.); Tsai CF ( Department of Applied Chemistry, National Chi Nan University, Puli, Nantou, 545 Taiwan.); Ho JA ( BioAnalytical Chemistry and Nanobiomedicine Laboratory, Department of Biochemical Science and Technology, National Taiwan University, Taipei, 10617 Taiwan.); Wu LC ( Department of Applied Chemistry, National Chi Nan University, Puli, Nantou, 545 Taiwan. Electronic address: lw25@ncnu.edu.tw.) |
| Abstract |
Tyramine (4-hydroxyphenethylamine), which is a monoamine metabolized by monoamine oxidase (MAO), exists widely in plants, animals, fermented foods, and salted foods. The incidence of hypertension, or 'cheese effect', which is associated with a large dietary intake of tyramine while taking MAO inhibitors has been reported; therefore, the measurement of tyramine is an urgent concern. Herein, an efficient approach that integrates a molecular imprinting polymer for solid phase extraction (MISPE) technique with a sensitive electrochemical sensing platform (SPCE/PEDOT: PSS/AuNP/1-m-4-MP) for the quantification of tyramine is presented. Enhanced electrode conductivity was achieved sequentially by constructing a conductive polymer (PEDOT: PSS) on a screen-printed carbon electrode (SPCE), followed by electrodeposition with gold nanoparticles (AuNPs) and, finally, by modification with positively charged 1-methyl-4-mercaptopyridine (1-m-4-MP) using an Au-S bond. Tyramine was isolated selectively and pre-concentrated by the MISPE technique; electroanalysis that used differential pulse voltammetry (DPV) in NaOH (0.1M, pH 13) was conducted successively. Experimental parameters (such as modes of electrode modification, ratio of PEDOT: PSS, pH of electrolyte, time required for AuNP deposition, and 1-m-4-MP concentrations) that were associated with optimal detection conditions were evaluated also. We obtained a linear concentration range (5-100nM, R =0.9939) with LOD and sensitivity at 2.31nM, and 3.11µAnM cm , respectively. The applicability of our technique was demonstrated by analyzing tyramine in spiked serum and milk. The feature of our newly developed analytical methods that coupled sample pre-treatment (sample clean-up and pre-concentration) with sensitive detection makes it a promising tool for quantifying of tyramine. |
| ISSN |
09565663 |
| Volume Number |
87 |
| e-ISSN |
18734235 |
| Journal |
Biosensors and Bioelectronics |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2017-01-15 |
| Publisher Place |
Great Britain (UK) |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Electrochemical Techniques Methods Milk Chemistry Molecular Imprinting Polystyrenes Solid Phase Extraction Thiophenes Tyramine Analysis Blood Animals Biosensing Techniques Instrumentation Carbon Electrodes Equipment Design Gold Humans Metal Nanoparticles Pyridines Evaluation Studies Journal Article Discipline Biotechnology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
