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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kang, Seong Ho Yeung, Edward S. Chakkarapani, Suresh Kumar Lee, Seungah |
| Description | Author Affiliation: Lee S ( Department of Applied Chemistry and Institute of Natural Sciences, College of Applied Science, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Republic of Korea.); Chakkarapani SK ( Department of Chemistry, Graduate School, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Republic of Korea.); Yeung ES ( Department of Chemistry, Iowa State University, Ames, IA 50011, USA.); Kang SH ( Department of Applied Chemistry and Institute of Natural Sciences, College of Applied Science, Kyung Hee University, Yongin-si, Gyeonggi-do 17104, Republic of Korea) |
| Abstract | Quantitative screening of influenza A (H7N9) virus without DNA amplification was performed based on single-particle dual-mode total internal reflection scattering (SD-TIRS) with a transmission grating (TG). A gold nanopad was utilized as a substrate for the hybridization of probe DNA molecules with the TIRS nanotag (silver-nanoparticle). The TG effectively isolated the scattering signals in first-order spectral images (n=+1) of the nanotag from that of the substrate, providing excellent enhancement of signal-to-noise and selectivity. By using single-DNA molecule/TIRS nanotag hybridization, target DNA molecules of H7N9 were detected down to 74 zM, which is at least 100,000 times lower than the current detection limit of 9.4fM. By simply modifying the design of the probe DNA molecules, this technique can be used to directly screen other viral DNAs in various human biological samples at the single-molecule level without target amplification. |
| ISSN | 09565663 |
| Volume Number | 87 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-01-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Dna, Viral Analysis Influenza A Virus, H7n9 Subtype Isolation & Purification Influenza, Human Virology Nucleic Acid Hybridization Instrumentation Dna Probes Chemistry Genetics Dna, Single-stranded Equipment Design Gold Humans Metal Nanoparticles Microscopy, Fluorescence Nanostructures Silver Journal Article Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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