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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhang, Peng Li, Xiaocheng Wei, Heng Men, Shuai Cui, Jingjing Liu, Hui Wang, Hongning Ma, Suzhen |
| Description | Author Affiliation: Zhang P ( School of Life Science, Sichuan University, Chengdu 610065, PR China); Liu H ( School of Life Science, Sichuan University, Chengdu 610065, PR China); Li X ( School of Life Science, Sichuan University, Chengdu 610065, PR China); Ma S ( School of Life Science, Sichuan University, Chengdu 610065, PR China); Men S ( School of Life Science, Sichuan University, Chengdu 610065, PR China); Wei H ( School of Life Science, Sichuan University, Chengdu 610065, PR China.); Cui J ( School of Life Science, Sichuan University, Chengdu 610065, PR China.); Wang H ( School of Life Science, Sichuan University, Chengdu 610065, PR China) |
| Abstract | The harm of Salmonella typhimurium (S. typhimurium) to public health mainly by the consumption of contaminated agricultural products or water stresses an urgent need for rapid detection methods to help control the spread of S. typhimurium. In this work, an intelligently designed sensor system took creative advantage of triple trigger sequences-regenerated strand displacement amplification and self-protective hairpin template-generated-scaffolded silver nanoclusters (AgNCs) for the first time. In the presence of live S. typhimurium, single-stranded trigger sequences were released from aptamer-trigger sequences complex, initiating a branch migration to open the hairpin template I containing complementary scaffolds of AgNCs. Then the first strand displacement amplification was induced to produce numerous scaffolds of AgNCs and reporter strands which initiated a branch migration to open the hairpin template II containing complementary scaffolds of AgNCs. Then the second strand displacement amplification was induced to generate numerous scaffolds of AgNCs and trigger sequences which initiated the third branch migration and strand displacement amplification to produce numerous scaffolds of AgNCs and reporter strands in succession. Cyclically, the reproduction of the trigger sequences and cascade successive production of scaffolds were achieved successfully, forming highly fluorescent AgNCs, thus providing significantly enhanced fluorescent signals to achieve ultrasensitive detection of live S. typhimurium down to 50 CFU/mL with a linear range from 10 to 10 CFU/mL. It is the first report on a fluorescent biosensor for detecting viable S. typhimurium directly, which can distinguish from heat denatured S. typhimurium. And it develops a new strategy to generate the DNA-scaffolds for forming AgNCs. |
| ISSN | 09565663 |
| Volume Number | 87 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-01-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Metal Nanoparticles Chemistry Poultry Microbiology Salmonella Typhimurium Isolation & Purification Silver Animals Aptamers, Nucleotide Chickens Fluorescent Dyes Humans Ultrastructure Salmonella Infections Spectrometry, Fluorescence Journal Article Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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