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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tanaka, Tomoyuki Li, Tian-Cheng Park, Enoch Y. Kitamoto, Noritoshi Ahmed, Syed Rahin Takemeura, Kenshin Suzuki, Tetsuro |
| Description | Author Affiliation: Ahmed SR ( Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: rahin_sust@yahoo.com.); Takemeura K ( Graduate School of Integrated Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: kenpi901@yahoo.co.jp.); Li TC ( Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-Murayama, Tokyo 208-0011, Japan. Electronic address: litc@nih.go.jp.); Kitamoto N ( School of Human Science and Environment, University of Hyogo, 1-1-12 Shinzaike-Honcho, Himeji, Hyogo 670-0092, Japan. Electronic address: kitamoto@shse.u-hyogo.ac.jp.); Tanaka T ( Hidaka General Hospital, Gobo, Wakayama 644-0002, Japan. Electronic address: moqui-tom@maia.eonet.ne.jp.); Suzuki T ( Department of Infectious Diseases, Hamamatsu University School of Medicine, 1-20-1 Higashi-ku, Handa-yama, Hamamatsu 431-3192, Japan. Electronic address: tesuzuki@hama-med.ac.jp.); Park EY ( Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan) |
| Abstract | A hybrid structure of graphene-gold nanoparticles (Grp-Au NPs) was designed as a new nanoprobe for colorimetric immunoassays. This hybrid structure was prepared using chloroauric acid, sodium formate and Grp flakes at room temperature. Au NPs attached strongly onto the Grp surface, and their size was controlled by varying the sodium formate concentration. The Raman intensity of the Grp-Au NP hybrids was significantly enhanced at 1567cm and 2730cm compared with those of pristine Grp because of the electronic interaction between Au NPs and Grp. The Grp-Au NPs with a hybrid structure catalyzed the oxidation of the peroxidase substrate 3,3,5,5-tetramethylbenzidine (TMB) with H O , developing a blue color in aqueous solution. This catalytic activity was utilized to detect norovirus-like particles (NoV-LPs) in human serum. The enhanced colorimetric response was monitored using Ab-conjugated-Grp-Au NPs and found to depend on the NoV-LP concentration, exhibiting a linear response from 100pg/mL to 10µg/mL. The limit of detection (LOD) of this proposed method was 92.7pg/mL, 112 times lower than that of a conventional enzyme-linked immunosorbent assay (ELISA). The sensitivity of this test was also 41 times greater than that of a commercial diagnostic kit. The selectivity of the Grp-Au NPs was tested with other viruses, and no color changes were observed. Therefore, the proposed system will facilitate the utilization of the intrinsic peroxidase-like activity of Grp-Au NPs in medical diagnostics. We believe that the engineered catalytic Grp-Au NP hybrids could find potential applications in the future development of biocatalysts and bioassays. |
| ISSN | 09565663 |
| Volume Number | 87 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2017-01-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Caliciviridae Infections Diagnosis Colorimetry Methods Gold Chemistry Graphite Nanoparticles Norovirus Isolation & Purification Biosensing Techniques Blood Virology Humans Limit Of Detection Ultrastructure Peroxidase Evaluation Studies Journal Article Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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