NDLI: Identification of DNA fragments carrying ecotropic proviruses of AKR mice.

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Identification of DNA fragments carrying ecotropic proviruses of AKR mice.

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Identification of DNA fragments carrying ecotropic proviruses of AKR mice.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Weinberg, R. A. Bird, S. Rowe, W. P. Steffen, D.
Abstract	The proviruses of the N-tropic, ecotropic virus (AKV) of AKR mice (Akv-1, Akv-2) have been studied by the Southern gel--filter transfer technique. These proviruses can be detected by cleavage of cell DNA by BamHI endonuclease, which yields characteristic subgenomic DNA fragments upon cleavage of this type of provirus. Proviruses integrated into different sites in the mouse genome can be resolved with EcoRI endonuclease, which does not cleave the AKV proviruses. Use of congenic and backcrossed mice and a radioactive DNA probe enriched for AKV sequences has allowed identification of the EcoRI fragments carrying the proviruses of the genetically defined Akv-1 and Akv-2 loci. Novel proviruses introduced by superinfection of cultured AKR cells with AKV and present in leukemic cells from AKR mice have also been identified. Comparison of substrains of AKR mice indicates some heterogeneity in their spectra of proviruses.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	9
Volume Number	76
Language	English
Publisher	National Academy of Sciences
Publisher Date	1979-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	AKR Murine Leukemia Virus Genetics Genes, Viral Leukemia Virus, Murine Leukemia, Experimental Mice, Inbred AKR Animals DNA Restriction Enzymes Genetic Linkage Mice Mice, Inbred Strains Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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