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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Baserga, R. Rossini, M. Weinmann, R. |
| Abstract | tsAF8 cells are a temperature-sensitive (ts) mutant of BHK cells that are arrested in G1 at the nonpermissive temperature. When made quiescent by serum restriction, they can be stimulated to enter S phase by 10% serum at 34 degrees C but not at 40.6 degrees C. The same results can be obtained if quiescent cells are infected with polyoma virus or adenovirus 12 instead of serum. However, adenovirus 2 infection stimulates DNA synthesis in tsAF8 cells at both 34 degrees C and 40.6 degrees C. The DNA synthesized after adenovirus 2 infection has been shown to be cellular DNA by CsCl density centrifugation. By density labeling it can be shown that adenovirus 2-induced DNA synthesis is due to semiconservative replication. The difference between adenovirus 2 and polyoma (or serum) is also evident with another ts mutant of BHK cells, ts13 cells. These results open the possibility of identifying the viral or cellular mechanism at the basis of this difference in the induction of host DNA synthesis between adenovirus 2 and polyoma or serum. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 9 |
| Volume Number | 76 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 1979-01-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Adenoviruses, Human Physiology Cell Cycle DNA Replication Polyomavirus Radiation Effects Animals Cell Line Cell Transformation, Viral Cricetinae Culture Media Drug Effects Dactinomycin Pharmacology Mutation Temperature Ultraviolet Rays Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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