NDLI: Cloning immunoglobulin gamma 2b chain gene of mouse: characterization and partial sequence determination.

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Photoelectric properties and detection of the aromatic carcinogens benza[a]pyrene and dimethylbenzanthracene.

Identification of mannose 6-phosphate in glycoproteins that inhibit the assimilation of β-galactosidase by fibroblasts

Cloning immunoglobulin gamma 2b chain gene of mouse: characterization and partial sequence determination.

Promoter mutations in the transfer RNA gene tyrT of Escherichia coli.

Passive modulation of blood-group antigens.

Immunoglobulin differentiation is dictated by repeated recombination sequences within the V region prototype gene: a hypothesis.

Some sequence similarities among cloned mouse DNA segments that code for lambda and kappa light chains of immunoglobulins.

Cloned pairs of variable region genes for immunoglobulin heavy chains isolated from a clone library of the entire mouse genome.

Cloning of the structural gene (ompA) for an integral outer membrane protein of Escherichia coli K-12.

Cloning of integrated Moloney sarcoma proviral DNA sequences in bacteriophage lambda.

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Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences.

Transcription promotes recA-independent recombination mediated by DNA-dependent RNA polymerase in Escherichia coli.

Sigma subunit of Escherichia coli RNA polymerase affects the function of lambda N gene.

Bacterial lipopolysaccharide activates suppressor B lymphocytes.

Photoaffinity labeling of cyclic-AMP- and AMP-binding proteins differentiating Dictyostelium discoideum cells.

Enzymatic methyl esterification of specific glutamyl residue in corticotropin.

Characterization of a histone-like protein extracted from yeast mitochondria.

Oncogenicity of simian virus 40 deletion mutants that induce altered 17-kilodalton t-proteins.

A general priming system employing only dnaB protein and primase for DNA replication.

Splicing as a requirement for biogenesis of functional 16S mRNA of simian virus 40.

Conserved primary sequences of the DNA terminal proteins of five different human adenovirus groups.

DNA synthesis in temperature-sensitive mutants of the cell cycle infected by polyoma virus and adenovirus.

Phospholipid methylation stimulates lactogenic binding in mouse mammary gland membranes.

Molecular genetics of herpes simplex virus: the terminal a sequences of the L and S components are obligatorily identical and constitute a part of a structural gene mapping predominantly in the S component.

Identification of DNA fragments carrying ecotropic proviruses of AKR mice.

Catabolite inactivation of gluconeogenic enzymes in mutants of yeast deficient in proteinase B.

Selective and unidirectional membrane redistribution of an H-2 antigen with an antibody-clustered viral antigen: relationship to mechanisms of cytotoxic T-cell interactions.

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Endogenous digitalis-like activity in mammalian brain.

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Characterization of an endopeptidase involved in pre-protein processing.

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Structure of genes for human growth hormone and chorionic somatomammotropin.

Enzymatic identification of mannose 6-phosphate on the recognition marker for receptor-mediated pinocytosis of beta-glucuronidase by human fibroblasts.

Identification of gamma-glutamyl phosphate in the alpha 2 chains of chicken bone collagen.

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Calculation of evolutionary trees from sequence data.

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Cytoplasmic inheritance of erythromycin resistance in human cells.

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DNA restriction endonuclease analysis for localization of human beta- and delta-globin genes on chromosome 11.

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Apolipoproteins in human cerebrospinal fluid.

Cholesterol homeostasis in the rat with a portacaval anastomosis.

Restriction endonuclease cleavage map and location of unique features of the DNA of hepatitis B virus, subtype adw2.

Regulation of the state of phosphorylation of specific neuronal proteins in mouse brain by in vivo administration of anesthetic and convulsant agents.

Target recognition in neurogenesis: formation of the Mauthner axon cap.

Localization of spontaneously hyperactive B cells of NZB mice to a specific B cell subset.

Monoclonal antibody directed to a B-cell antigen present in rats, mice, and humans.

'Hybrid' synapses formed by foreign innervation of parasympathetic neurons: a model for selectivity during competitive reinnervation.

Differences in mutagenicity and cytotoxicity of (+)- and (-)-benzo[a]pyrene 4,5-oxide: a synergistic interaction of enantiomers.

Yeast chromatin is uniformly digested by DNase-I.

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Energy-dependent processing of cytoplasmically made precursors to mitochondrial proteins.

Subunit structure of dihydrolipoyl transacetylase component of pyruvate dehydrogenase complex from Escherichia coli.

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Selective loss of noradrenergic phenotypic characters in neuroblasts of the rat embryo.

Conformation of gramicidin A channel in phospholipid vesicles: a 13C and 19F nuclear magnetic resonance study.

Characterization of alkylamine-sensitive site in alpha 2-macroglobulin.

Trace polypeptides in cellular extracts and human body fluids detected by two-dimensional electrophoresis and a highly sensitive silver stain.

Synthesis of a 600-nucleotide-long plus-strand DNA by virions of Moloney murine leukemia virus.

Comparison of amino acid sequences of two human histocompatibility antigens, HLA-A2 and HLA-B7: location of putative alloantigenic sites.

Differentiation of 3T3-L2 fibroblasts into adipose cells in bromodeoxyuridine-suppressed cultures.

Identification of a Rous sarcoma virus transformation-related protein in normal avian and mammalian cells.

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Cloning immunoglobulin gamma 2b chain gene of mouse: characterization and partial sequence determination.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Honjo, T. Yamagishi, H. Kataoka, T. Yamawaki-kataoka, Y.
Abstract	DNA from newborn mice was digested with restriction endonuclease EcoRI, and a 6.6-kilobase fragment encoding immunoglobulin gamma 2b chain mRNA derived from MPC 11 myeloma was enriched about 100-fold by RPC-5 column chromatography and agarose gell electrophoresis. The 6.6-kilobase fragment was cloned with lambda gt WES.lambda B as EK2 vector. The cloned phage (lambda WES.IgH22) contained the constant region gene of the gamma 2b chain but not the variable region gene of MPC 11 mRNA. The constant region genes of the other gamma chains (i.e., gamma 1, gamma 2a, and gamma 3) were not present in lambda gt WES.IgH22 DNA. R-loop mapping indicates that the gamma 2b chain structural gene is divided into two parts (330 +/- 60 SD base pairs and 930 +/- 110 SD base pairs) by an intervening sequence (360 +/- 100 SD base pairs). The nucleotide sequence around the junction of the hinge region and CH2 domain was determined and shown to match the amino acid sequence of the initial part of the CH2 domain of the gamma 2b chain. The base sequence upstream from the junction, however, is unrelated to the amino acid sequence of the CH1 domain and the hinge region of all the gamma chains whose sequences have been determined. These results indicate that the gamma 2b chain gene is interrupted at the junction of the hinge region and CH2 domain by an intervening sequence. The existence of two more intervening sequences, one between the CH1 domain and the hinge region and the other between the CH2 and CH3 domains, is discussed.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	9
Volume Number	76
Language	English
Publisher	National Academy of Sciences
Publisher Date	1979-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Genes Immunoglobulin Heavy Chains Genetics Immunoglobulin Gamma-Chains Animals Codon DNA, Recombinant Mice Nucleic Acid Hybridization RNA, Messenger Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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