NDLI: Non-plasma bonding of PDMS for inexpensive fabrication of microfluidic devices.

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Non-plasma bonding of PDMS for inexpensive fabrication of microfluidic devices.

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Non-plasma bonding of PDMS for inexpensive fabrication of microfluidic devices.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Harris, Joseph Lee, Hyuna Vahidi, Behrad Tu, Cristina Cribbs, David Cotman, Carl Jeon, Noo Li
Description	Country affiliation: United States Author Affiliation: Harris J ( Department of Biomedical Engineering, University of California, Irvine, CA, USA. jwharris7@gmail.com)
Abstract	In this video, we demonstrate how to use the neuron microfluidic device without plasma bonding. In some cases it may be desirable to reversibly bond devices to the Corning No. 1 cover glass. This could be due, perhaps, to a plasma cleaner not being available. In other instances, it may be desirable to remove the device from the glass after the culturing of neurons for certain types of microscopy or for immunostaining, though it is not necessary to remove the device for immunostaining since the neurons can be stained in the device. Some researchers, however, still prefer to remove the device. In this case, reversible bonding of the device to the cover glass makes that possible. There are some disadvantages to non-plasma bonding of the devices in that not as tight of a seal is formed. In some cases axons may grow under the grooves rather than through them. Also, because the glass and PDMS are hydrophobic, liquids do not readily enter the device making it necessary at times to force media and other reagents into the device. Liquids will enter the device via capillary action, but it takes significantly longer as compared to devices that have been plasma bonded. The plasma cleaner creates temporary hydrophilic charges on the glass and device that facilitate the flow of liquids through the device after bonding within seconds. For non-plasma bound devices, liquid flow through the devices takes several minutes. It is also important to note that the devices to be used with non-plasma bonding need to be sterilized first, whereas plasma treated devices do not need to be sterilized prior to use because the plasma cleaner will sterilize them.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/410
Journal	Journal of Visualized Experiments
Issue Number	9
Language	English
Publisher	MyJove Corp.
Publisher Date	2007-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Dimethylpolysiloxanes Microfluidic Analytical Techniques Instrumentation Neurons Cytology Nylons Animals Video-audio Media
Content Type	Text
Resource Type	Article

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