NDLI: Evaluation of antiproliferative and antioxidant activities of the organic extract and its polar fractions from the Mediterranean gorgonian Eunicella singularis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Deghrigue, Monia Dellai, Afef Akremi, Najoua Le Morvan, Valérie Robert, Jacques Bouraoui, Abderrahman
Spatial Coverage	Mediterranean Sea
Description	Country affiliation: Tunisia Author Affiliation: Deghrigue M ( Unité de Recherche des Substances Actives Marines (URSAM), Laboratoire de Pharmacologie, Faculté de pharmacie, Université de Monastir, Tunisia. moniadeghrigue@yahoo.fr)
Abstract	The present study was conducted to evaluate the antiproliferative and antioxidant activities of organic extract and its polar fractions from Eunicella singularis (Esper 1794). Organic extract and two fractions of E. singularis (F2 and F3) were screened for the presence of phenolic compounds, terpenoids and glycosides. The antiproliferative activity of E. singularis organic extract and its polar fractions was evaluated on human cancer cell lines (A549, lung cell carcinoma; HCT15, colon cell carcinoma and MCF7, breast adenocarcinoma), using the MTT colorimetric method and clonogenic assay, as well as the antioxidant activity, using the stable radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), and the FRAP assays. The fractions F2 and F3 showed significant total phenolic content (40 and 35.72mg gallic-acid equivalent/g dried sample), respectively, and important antiproliferative properties against the cancer cell lines. The IC50 values, ranged from 36 to 274µg/ml for A549; 93 to 426µg/ml for HCT15; and 52 to 225µg/ml for MCF7 and in the clonogenic inhibition assay from 18 to 134µg/ml for A549; 43 to 357µg/ml for HCT15; and 17 to 160µg/ml for MCF7. Using the DPPH method, the fraction F2 exhibited the strongest radical scavenging activity, with IC50 0.08mg/ml, which approaches the activity of the powerful antioxidant standard, ascorbic acid (IC50=0.064mg/ml). The reducing power of the samples was in the following order: F2>organic extract>F3. These results suggest that E. singularis fractions might be used as a potential source of natural antioxidant and antitumor agents. The purification and determination of the chemical structures of compounds in these active fractions are under investigation. The results could provide a compound(s) with a promising role in future medicines.
File Format	HTM / HTML
ISSN	13826689
Issue Number	2
Volume Number	36
e-ISSN	18727077
Journal	Environmental Toxicology and Pharmacology
Language	English
Publisher	Elsevier
Publisher Date	2013-09-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Environmental Health Discipline Pharmacology Antineoplastic Agents Pharmacology Antioxidants Cell Proliferation Drug Effects Cnidaria Chemistry Solvents Animals Isolation & Purification Biphenyl Compounds Cell Survival Dose-response Relationship, Drug Glycosides Inhibitory Concentration 50 Mcf-7 Cells Mediterranean Sea Phenols Picrates Terpenes Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Medicine Toxicology Pharmacology

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