NDLI: Molecular cloning and expression of the gene for G protein alpha subunit induced by bisphenol A in marine polychaete Perinereis aibuhitensis.

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Molecular cloning and expression of the gene for G protein alpha subunit induced by bisphenol A in marine polychaete Perinereis aibuhitensis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhao, Huan Zhou, Yibing Li, Ying Li, Shaojuan Yang, Dazuo
Description	Country affiliation: China Author Affiliation: Zhao H ( Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning province, Dalian Ocean University, Dalian, 116023, China.); Zhou Y ( Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning province, Dalian Ocean University, Dalian, 116023, China. Electronic address: ybzhou2011@hotmail.com.); Li Y ( Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning province, Dalian Ocean University, Dalian, 116023, China.); Li S ( Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning province, Dalian Ocean University, Dalian, 116023, China.); Yang D ( Key Laboratory of Marine Bio-resources Restoration and Habitat Reparation in Liaoning province, Dalian Ocean University, Dalian, 116023, China.)
Abstract	A G protein alpha subunit gene named Pa G was isolated from the marine polychaete Perinereis aibuhitensis. The full-length cDNA of Pa G was 1832 bp and contained a 205 bp 5' untranslated region (5'UTR), a 565 bp 3' UTR and a 1062 bp open reading frame encoding 353 amino acid residues. The deduced protein sequence of Pa G showed 73% homology with the G protein of Dipolydora quadrilobata. Tissue-specific expression induced by exposure to bisphenol A (BPA) in P. aibuhitensis was detected by real-time PCR, which showed BPA induced expression of the Pa G gene, and the level of transcription was related positively to the concentration of BPA and the length of exposure time. With increasing concentration of BPA and length of exposure time, the level of mRNA transcription was raised gradually, but the level of increasing expression of Pa G mRNA induced by exposure to BPA varied significantly among different tissues.
File Format	HTM / HTML
ISSN	13826689
Issue Number	2
Volume Number	37
e-ISSN	18727077
Journal	Environmental Toxicology and Pharmacology
Language	English
Publisher	Elsevier
Publisher Date	2014-03-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Environmental Health Discipline Pharmacology Benzhydryl Compounds Toxicity Gtp-binding Protein Alpha Subunits Genetics Phenols Polychaeta Water Pollutants, Chemical Amino Acid Sequence Animals Base Sequence Cloning, Molecular Dna, Complementary Gene Expression Molecular Sequence Data Rna, Messenger Metabolism Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Medicine Toxicology Pharmacology

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