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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tu, Hongtao Qin, Yuchuan |
| Description | Country affiliation: China Author Affiliation: Tu H ( Department of Entomology, China Agricultural University, Beijing, China) |
| Abstract | The heterotrimeric G proteins play an essential role in a wide variety of signal transduction pathways, mediating the process of chemical signals from the environment in all higher eukaryotic organisms. In this article, two G-protein subunit genes encoding G q and Gß1 were cloned from Bemisia tabaci Gennadius. The full-length cDNA sequence of BtG q consisted of 2,336 bp with an ORF of 1,062 bp encoding 353 amino acids and BtGß1 had a full length of 1,942 bp with an ORF of 1,023 nucleotides encoding 340 amino acids. The amino acid sequences of BtG q and BtGß1 from B. tabaci B biotype were identical to those from the Q biotype. Phylogenetic analysis identified G protein and ß subunit families from insects based on their amino acid sequences. The expression patterns of BtG q and BtGß1 at different development stages and in different body regions were analyzed by real-time quantitative PCR and Western blot. The results show that BtG q and BtGß1 are neither developmental stage-specific nor tissue-specific. The transcript levels of BtG q in the B biotype are similar to that in the Q biotype, the transcript levels of BtGß1 at egg, first instar and pupae in B biotype were significantly higher than that in Q biotype. The transcript levels of BtG q and BtGß1 in the head were significantly higher than those in thorax and abdomen indicating that they are involved in nervous system and sensory functions. |
| File Format | HTM / HTML |
| ISSN | 07394462 |
| Issue Number | 2 |
| Volume Number | 87 |
| e-ISSN | 15206327 |
| Journal | Archives of Insect Biochemistry and Physiology |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 2014-10-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Physiology Discipline Biochemistry Discipline Zoology Gtp-binding Protein Alpha Subunits, Gq-g11 Genetics Gtp-binding Protein Beta Subunits Hemiptera Amino Acid Sequence Animals Base Sequence Blotting, Western Cloning, Molecular Female Biosynthesis Gene Expression Regulation, Developmental Metabolism Male Molecular Sequence Data Phylogeny Rna Chemistry Real-time Polymerase Chain Reaction Sequence Alignment Sequence Analysis, Dna Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Medicine Biochemistry Insect Science |
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