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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bednarek, K. Kiwerska, K. Szaumkessel, M. Bodnar, M. Kostrzewska-Poczekaj, M. Marszalek, A. Janiszewska, J. Bartochowska, A. Jackowska, J. Wierzbicka, M. Grenman, R. Szyfter, K. Giefing, M. Jarmuz-Szymczak, M. |
| Description | Country affiliation: Poland Author Affiliation: Bednarek K ( Department of Cancer Genetics, Institute of Human Genetics, PAS, Poznan, Poland.); Kiwerska K ( Department of Cancer Genetics, Institute of Human Genetics, PAS, Poznan, Poland.); Szaumkessel M ( Department of Cancer Genetics, Institute of Human Genetics, PAS, Poznan, Poland.); Bodnar M ( Department of Clinical Pathomorphology, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland.); Kostrzewska-Poczekaj M ( Department of Cancer Genetics, Institute of Human Genetics, PAS, Poznan, Poland.); Marszalek A ( Department of Clinical Pathomorphology, Collegium Medicum, Nicolaus Copernicus University, Bydgoszcz, Poland.); Janiszewska J ( Department of Oncologic Pathology, Greater Poland Cancer Centre, Poznan, Poland.); Bartochowska A ( Department of Cancer Genetics, Institute of Human Genetics, PAS, Poznan, Poland.); Jackowska J ( Department of Otolaryngology and Laryngological Oncology, University of Medical Sciences, Poznan, Poland.); Wierzbicka M ( Department of Otolaryngology and Laryngological Oncology, University of Medical Sciences, Poznan, Poland.); Grenman R ( Department of Otolaryngology and Laryngological Oncology, University of Medical Sciences, Poznan, Poland.); Szyfter K ( Department of Otorhinolaryngology-Head and Neck Surgery and Department of Medical Biochemistry, Turku University Hospital and University of Turku, Turku, Finland.); Giefing M ( Department of Audiology and Phoniatry, University of Medical Sciences, Poznan, Poland.); Jarmuz-Szymczak M ( Department of Cancer Genetics, Institute of Human Genetics, PAS, Poznan, Poland.) |
| Abstract | In this study, we analyzed the expression profile of four genes (CCNA2, CCNB1, CCNB2, and CDK1) in laryngeal squamous cell carcinoma (LSCC) cell lines and tumor samples. With the application of microarray platform, we have shown the overexpression of these genes in all analyzed LSCC samples in comparison to non-cancer controls from head and neck region. We have selected CDK1 for further analysis, due to its leading role in cell cycle regulation. It is a member of the Ser/Thr protein kinase family of proven oncogenic properties. The results obtained for CDK1 were further confirmed with the application of reverse transcription quantitative polymerase chain reaction (RT-qPCR) technique, Western blot, and immunohistochemistry (IHC). The observed upregulation of CDK1 in laryngeal squamous cell carcinoma has encouraged us to analyze for genetic mechanisms that can be responsible this phenomenon. Therefore, with the application of array-CGH, sequencing analysis and two methods for epigenetic regulation analysis (DNA methylation and miRNA expression), we tried to identify such potential mechanisms. Our attempts to identify the molecular mechanisms responsible for observed changes failed as we did not observe significant alterations neither in the DNA sequence nor in the gene copy number that could underline CDK1 upregulation. Similarly, the pyrosequencing and miRNA expression analyses did not reveal any differences in methylation level and miRNA expression, respectively; thus, these mechanisms probably do not contribute to elevation of CDK1 expression in LSCC. However, our results suggest that alteration of CDK1 expression on both mRNA and protein level probably appears on the very early step of carcinogenesis. |
| File Format | HTM / HTML |
| ISSN | 10104283 |
| e-ISSN | 14230380 |
| DOI | 10.1007/s13277-016-4991-4 |
| Journal | Tumor Biology |
| Issue Number | 8 |
| Volume Number | 37 |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2016-08-01 |
| Publisher Place | Netherlands |
| Access Restriction | Open |
| Subject Keyword | Tumour Cancer Oncology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Cancer Research |
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