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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Huang, Wenbai Liu, Zhan'ao Zhou, Guanzhou Ling, Jianmin Tian, Ailing Sun, Nianfeng |
| Description | Author Affiliation: Huang W ( Department of General Surgery, Qilu Hospital of Shandong University, No. 107 West Wenhua Road, Jinan, Shandong, 200012, People's Republic of China.); Liu Z ( School of Medicine, Shandong University, No. 44 West Wenhua Road, Jinan, Shandong, 200012, People's Republic of China.); Zhou G ( Department of General Surgery, Qilu Hospital of Shandong University, No. 107 West Wenhua Road, Jinan, Shandong, 200012, People's Republic of China.); Ling J ( School of Medicine, Shandong University, No. 44 West Wenhua Road, Jinan, Shandong, 200012, People's Republic of China.); Tian A ( Department of General Surgery, Qilu Hospital of Shandong University, No. 107 West Wenhua Road, Jinan, Shandong, 200012, People's Republic of China.); Sun N ( School of Medicine, Shandong University, No. 44 West Wenhua Road, Jinan, Shandong, 200012, People's Republic of China.) |
| Abstract | Apoptosis disorder is generally regarded as an important mechanism of carcinogenesis. Inducement of tumor cell apoptosis can be an effectual way to treat cancer. Bcl-2-associated athanogene 1 (Bag-1) is a positive regulator of Bcl-2 which is an anti-apoptotic gene. Bag-1 is highly expressed in colorectal cancer, which plays a critical role in promoting metastasis, poor prognosis, especially in anti-apoptotic function, and is perhaps a valuable gene target for colorectal cancer therapy. Recently, we applied a novel non-viral gene carrier, magnetic gold nanoparticle, and mediated plasmid pGPH1/GFP/Neo-Bag-1-homo-825 silencing Bag-1 gene for treating colorectal cancer in vivo and in vitro. By mediating with magnetic gold nanoparticle, siRNA plasmid was successfully transfected into cell. In 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, magnetic gold nanoparticle had no significant cytotoxicity and by which delivered RNA plasmid inhibited cell viability significantly (P < 0.05). Downregulation of Bag-1 promoted cell apoptosis (â¼47.0 %) in vitro and significantly decreased tumor growth when the cells were injected into nude mice. Based on the studies in vivo, the relative expression of Bag-1 was 0.165 ± 0.072 at mRNA level and â¼60 % at protein level. In further study, C-myc and ß-catenin, mainly molecules of Wnt/ß-catenin pathway, were decreased notably when Bag-1 were silenced in nanoparticle plasmid complex-transfected Balb c/nude tumor xenograft. In conclusion, Bag-1 is confirmed an anti-apoptosis gene that functioned in colorectal cancer, and the mechanism of Bag-1 gene causing colorectal cancer may be related to Wnt/ß-catenin signaling pathway abnormality and suggested that magnetic gold nanoparticle-delivered siRNA plasmid silencing Bag-1 is an effective gene therapy method for colorectal cancer. |
| File Format | HTM / HTML |
| ISSN | 10104283 |
| Issue Number | 8 |
| Journal | Tumor Biology |
| Volume Number | 37 |
| e-ISSN | 14230380 |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2016-08-01 |
| Publisher Place | Netherlands |
| Access Restriction | Subscribed |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Cancer Research |
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