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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wu, Di-di Li, Xue-Song Meng, Xiao-Na Yan, Jing Zong, Zhi-Hong |
| Description | Author Affiliation: Wu DD ( Department of Biochemistry and Molecular Biology, College of Basic Medicine, China Medical University, Shenyang, 110013, People's Republic of China.); Li XS ( Department of Biochemistry and Molecular Biology, College of Basic Medicine, China Medical University, Shenyang, 110013, People's Republic of China.); Meng XN ( Department of Biochemistry and Molecular Biology, College of Basic Medicine, China Medical University, Shenyang, 110013, People's Republic of China.); Yan J ( Department of Biochemistry and Molecular Biology, College of Basic Medicine, China Medical University, Shenyang, 110013, People's Republic of China.); Zong ZH ( Department of Biochemistry and Molecular Biology, College of Basic Medicine, China Medical University, Shenyang, 110013, People's Republic of China. zongzhi_999@163.com.) |
| Abstract | Ovarian cancer is commonly treated with cisplatin and paclitaxel combination chemotherapy; however, ovarian cancer cells often develop resistance to these drugs. Increasingly, microRNAs (miRNAs) including miR-873 have been implicated in drug resistance in many cancers, but the role of miR-873 in ovarian cancer remains unknown. MTT cell viability assays revealed that the sensitivities of ovarian cancer lines to cisplatin and paclitaxel increased following transfection with miR-873 (P < 0.05). After predicting the miR-873 binding region in the 3'-untranslated region of ABCB1, dual-luciferase reporter assay confirmed this prediction. RT-PCR and Western blotting revealed that MDR1 expression was significantly downregulated after transfection with miR-873 and upregulated after transfection with anti-miR-873 at both mRNA and protein levels compared to negative controls (P < 0.05). Experiments in a mouse xenograft model confirmed that intratumoral administration of miR-873 could enhance the efficacy of cisplatin in inhibiting tumor growth in ovarian cancer in vivo (P < 0.05). ABCB1 overexpression reduced sensitivities of ovarian cancer lines OVCAR3 and A2780 to cisplatin and paclitaxel, which can be reversed by miR-873 mimic transfection (P < 0.05). In summary, we demonstrated that overexpression of miR-873 increased the sensitivity of ovarian cancer cells to cisplatin and paclitaxel by targeting MDR1 expression. Our findings suggest that combination therapies with chemotherapy agents and miR-873 may suppress drug resistance in ovarian cancer. |
| File Format | HTM / HTML |
| ISSN | 10104283 |
| Issue Number | 8 |
| Journal | Tumor Biology |
| Volume Number | 37 |
| e-ISSN | 14230380 |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2016-08-01 |
| Publisher Place | Netherlands |
| Access Restriction | Subscribed |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Cancer Research |
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