NDLI: Pancreatic islet derived stem cells can express co-stimulatory molecules of antigen-presenting cells.

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Factors associated with sustained virological response in liver transplant recipients with recurrent hepatitis C.

Delayed kidney allograft function after simultaneous pancreas-kidney transplantation.

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Pancreatic islet derived stem cells can express co-stimulatory molecules of antigen-presenting cells.

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A lucky fall? Case report.

Simultaneous pancreas-kidney transplantation in HIV-infected patients: a case report and literature review.

Late-onset intestinal perforation in the setting of posttransplantation microangiopathy: a case report.

Treatment of acute graft-vs-host disease after simultaneous pancreas-kidney transplantation: a case report.

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Pancreatic islet derived stem cells can express co-stimulatory molecules of antigen-presenting cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Karaoz, E. Okçu, A. Saglam, O. Genc, Z. Seda Ayhan, S. Kasap, M.
Description	Country affiliation: Turkey Author Affiliation: Karaoz E ( Center for Stem Cell and Gene Therapies Research and Practice, Institute of Health Sciences, Department of Stem Cell, Kocaeli University, Kocaeli, Turkey. ekaraoz@hotmail.com)
Abstract	BACKGROUND: Antigen-presenting cells (APCs) are crucial intermediates in the generation of both innate and specific immune responses. It has long been understood that some APCs are resident in islets in situ as well as after isolation. Our aim was to investigate the presence of molecules involved in antigen presentation in rat pancreatic islet-derived stem cells (PI-SCs). METHODS: We used immunocytochemistry and reverse transcription polymerization chain reaction to study immunophenotypic characteristics; pluripotent-related gene expressions; transcripts coding for antigen-presenting surface proteins CD40, CD80, CD86; and major histocompatibility complex class II in addition to genes with known antiapoptotic functions including mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2), tumor necrosis factor alpha-induced protein 3 (TNFAIP3) interacting protein 1 (TNIP1) and BCL3 of the PI-SCs. RESULTS: Rat PI-SCs were negative for CD45 as demonstrated by flow cytometry and for CD31, CD34, and CD71 as demonstrated by immunocytochemistry. Therefore, there was no evidence of hematopoietic precursors in the cultures. OCT4, SOX2, and REX1 were expressed by rat PI-SCs. We determined the expression of genes for antigen-presenting surface proteins CD40 and CD80, and genes with known antiapoptotic functions including MAPKAPK2, TNIP1 and BCL3, besides the surface protein, CD80, by flow cytometry. CONCLUSION: Expression of these genes by rat PI-SCs implied that they could be involved in the regulation of immunity in islets, highlighting the influence of protective role-playing antiapoptotic mechanisms on pancreatic islet cells. This study offers the potential to understand the molecular mechanisms of a devastating disease, type-1 diabetes mellitus.
File Format	HTM / HTML
ISSN	00411345
Issue Number	9
Volume Number	42
e-ISSN	18732623
Journal	Transplantation Proceedings
Language	English
Publisher	Elsevier
Publisher Date	2010-11-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Transplantation__semicolon__medicine Antigen-presenting Cells Immunology Islets Of Langerhans Pluripotent Stem Cells Stem Cells Animals Antigens, Surface Genetics Metabolism Apoptosis Regulatory Proteins Biological Markers Cells, Cultured Flow Cytometry Gene Expression Regulation Immunohistochemistry Immunophenotyping Cytology Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Transplantation Surgery

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