NDLI: Expansion and differentiation of germline-derived pluripotent stem cells on biomaterials.

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Expansion and differentiation of germline-derived pluripotent stem cells on biomaterials.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hoss, Mareike Saric, Tomo Denecke, Bernd Peinkofer, Gabriel Bovi, Manfred Groll, Jürgen Ko, Kinarm Salber, Jochen Halbach, Marcel Schöler, Hans R. Zenke, Martin Neuss, Sabine
Description	Country affiliation: Germany Author Affiliation: Hoss M ( Interdisciplinary Centre for Clinical Research Aachen IZKF Aachen, RWTH Aachen University, Aachen 52074, Germany. mareike.hoss@rwth-aachen.de)
Abstract	Stem cells with broad differentiation potential, such as the recently described germline-derived pluripotent stem cells (gPS cells), are an appealing source for tissue engineering strategies. Biomaterials can inhibit, support, or induce proliferation and differentiation of stem cells. Here we identified (1) polymers that maintain self-renewal and differentiation potential of gPS cells for feeder-free expansion and (2) polymers supporting the cardiomyogenic fate of gPS cells by analyzing a panel of polymers of an established biomaterial bank previously used to assess growth of diverse stem cell types. Identification of cytocompatible gPS cell/biomaterial combinations required analysis of several parameters, including morphology, viability, cytotoxicity, apoptosis, proliferation, and differentiation potential. Pluripotency of gPS cells was visualized by the endogenous Oct4-promoter-driven GFP and by Sox2 and Nanog immunofluorescence. Viability assay, proliferation assay, and flow cytometry showed that gPS cells efficiently adhere and are viable on synthetic polymers, such as Resomer(®) LR704 (poly(L-lactic-D,L-lactic acid), poly(tetrafluor ethylene) (PTFE), poly(vinylidene fluoride) (PVDF), and on gelatine-coated tissue culture polystyrene. Expansion experiments showed that Resomer LR704 is an alternative substrate for feeder-free gPS cell maintenance. Resomer LR704, PTFE, and PVDF were found to be suitable for gPS cell differentiation. Spontaneous beating in embryoid bodies cultured on Resomer LR704 occurred already on day 8 of differentiation, much earlier compared to the other surfaces. This indicates that Resomer LR704 supports spontaneous cardiomyogenic differentiation of gPS cells, which was also confirmed on molecular, protein and functional level.
File Format	HTM / HTML
ISSN	21524947
e-ISSN	21524955
DOI	10.1089/ten.TEA.2012.0185
Journal	Tissue Engineering Part A
Issue Number	9-10
Volume Number	19
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2013-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Biotechnology Biocompatible Materials Chemistry Pluripotent Stem Cells Cytology Animals Cell Differentiation Physiology Cell Proliferation Cells, Cultured Flow Cytometry Germ Cells Immunohistochemistry Lactic Acid Mice Microscopy, Electron, Scanning Metabolism Polyesters Polymers Polytetrafluoroethylene Polyvinyls Reverse Transcriptase Polymerase Chain Reaction Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article

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