NDLI: The clinical importance of flow cytometry crossmatch in the context of CDC crossmatch results.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Graff, R. J. Buchanan, P. M. Dzebisashvili, N. Schnitzler, M. A. Tuttle-Newhall, J. Xiao, H. Schadde, E. Gheorghian, A. Lentine, K. L.
Spatial Coverage	United States
Description	Country affiliation: United States Author Affiliation: Graff RJ ( Department of Surgery, HLA Laboratory, Saint Louis University, St. Louis, Missouri, USA.)
Abstract	BACKGROUND: The complement-dependent microcytotoxicity crossmatch (CDCXM) is a standard method for evaluating the presence of preformed antibodies before transplantation. The flow cytometry crossmatch (FCXM) is more sensitive, but there is controversy regarding translation of its increased sensitivity to clinically relevant graft outcomes. METHODS: We analyzed Organ Procurement and Transplant Network registry data for living and deceased donor kidney transplants performed in 1995 to 2009 after both CDCXM and FCXM testing. Transplants with negative CDCXM (CDCXM(-)) and with T-cell positive (T(+)), T-cell negative/B-cell positive (T(-)B(+)), or T- and B-cell negative (T(-)B(-)) FCXM results were included. Graft survival according to crossmatch results was compared by survival analysis. RESULTS: Among patients transplanted with negative CDCXM (CDCXM(-)), deceased and living donor graft recipients with T(+) FXCM experienced significant absolute reductions in 5-year graft survival of 11.5% and 8.8% compared to those with T(-) FCXM (P < .0001). Compared to patients with FCXM(-)/CDCXM(-) deceased and living donor recipients with T(-)B(+) FCXM/CDCXM(-) had absolute reductions in 5-year graft survival of 9.6% and 7.6%, respectively (P < .0001). Upon multivariate adjustment with Cox regression, T(+) FCXM/CDCXM(-) deceased donor transplantation was associated with 51% higher adjusted relative risk of 1-year graft loss than FCXM(-)/CDCXM(-). Relative risks were more marked at 1 year for the T(+) groups but stronger in the 1- to 5-year interval for the T(-)B(+) groups. CONCLUSION: Positive FCXM has important prognostic implications even when CDCXM is negative. Thus, positive FCXM should not routinely be dismissed as 'overly sensitive' when CDCXM is negative.
File Format	HTM / HTML
ISSN	00411345
Issue Number	9
Volume Number	42
e-ISSN	18732623
Journal	Transplantation Proceedings
Language	English
Publisher	Elsevier
Publisher Date	2010-11-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Transplantation__semicolon__medicine Antibodies Blood Cytotoxicity Tests, Immunologic Flow Cytometry Graft Rejection Immunology Graft Survival Histocompatibility Testing Methods Kidney Transplantation Adverse Effects B-lymphocytes Humans Kaplan-meier Estimate Living Donors Predictive Value Of Tests Proportional Hazards Models Registries Risk Assessment Risk Factors Sensitivity And Specificity T-lymphocytes Time Factors Tissue And Organ Procurement Treatment Outcome United States Journal Article Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Transplantation Surgery

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Pretransplant donor-specific HLA antibodies detected by single antigen bead flow cytometry: risk factors and outcomes after kidney transplantation.

Late graft loss among pediatric recipients of DCD kidneys.

Cytotoxic flow cytometric crossmatch in renal transplantation: a single assay to simultaneously detect antibody binding and cytotoxicity.

Impact of flow cytometry crossmatch B-cell positivity on living renal transplantation.

Factors affecting graft and patient survival after live donor kidney transplantation in the UK.

Modified flow cytometry crossmatch detecting alloantibody-related cytotoxicity as a way to distinguish lytic antibodies from harmless in allosensitised kidney recipients.

Does lymphocyte cross-matching predict acute rejection and graft survival in liver transplantation?

Outcomes in partial liver transplantation: deceased donor split-liver vs. live donor liver transplantation.

Long-term results after lung transplantation using organs from circulatory death donors: a propensity score-matched analysis.

The clinical importance of flow cytometry crossmatch in the context of CDC crossmatch results.

Similar Documents

Pretransplant donor-specific HLA antibodies detected by single antigen bead flow cytometry: risk factors and outcomes after kidney transplantation.

Late graft loss among pediatric recipients of DCD kidneys.

Cytotoxic flow cytometric crossmatch in renal transplantation: a single assay to simultaneously detect antibody binding and cytotoxicity.

Impact of flow cytometry crossmatch B-cell positivity on living renal transplantation.

Factors affecting graft and patient survival after live donor kidney transplantation in the UK.

Modified flow cytometry crossmatch detecting alloantibody-related cytotoxicity as a way to distinguish lytic antibodies from harmless in allosensitised kidney recipients.

Does lymphocyte cross-matching predict acute rejection and graft survival in liver transplantation?

Outcomes in partial liver transplantation: deceased donor split-liver vs. live donor liver transplantation.

Long-term results after lung transplantation using organs from circulatory death donors: a propensity score-matched analysis.

The clinical importance of flow cytometry crossmatch in the context of CDC crossmatch results.

Long-term results after lung transplantation using organs from circulatory death donors: a propensity score-matched analysis.