NDLI: Prognostication of OCT4 isoform expression in prostate cancer

Content Provider	Springer Nature Link
Author	Resende, Marina França Chinen, Ludmilla Thomé Domingos Vieira, Samantha Jampietro, Juliano Fonseca, Francisco Paulo Vassallo, José Campos, Luciene Cristina Guimarães, Gustavo Cardoso Soares, Fernando Augusto Rocha, Rafael Malagoli
Copyright Year	2013
Abstract	Cancer stem cells (CSCs) refer to a subset of tumor cells that self-renew and affect tumor heterogeneity. This model has attracted considerable interest in recent years due to its implications in the prognosis and clinical management of cancer because CSCs mediate the occurrence, growth, and recurrence of tumors. OCT4 is central to embryonic stem cell self-renewal and differentiation into specific lineages and encodes two chief isoforms that are generated by alternative splicing—OCT4A and OCT4B. Their function in prostate cancer (PCa) is unknown. The prognostic function of OCT4 isoforms in PCa samples was examined by immunohistochemistry (IHC) and sensitivity and specificity of the antibodies used were evaluated by molecular biology techniques. Biochemical and pathological data and specimens from 193 patients with PCa were evaluated retrospectively. IHC, western blot, immunofluorescence, and automated image analysis were also performed. IHC was performed on a tissue microarray, and western blot and immunofluorescence were performed using the PCa cell line DU-145. IHC expression of OCT4 isoforms correlated with biochemical and pathological parameters, particularly biochemical recurrence-free survival (BCRFS). Patients with higher levels of OCT4B had lower Gleason scores and decreased likelihood of experiencing biochemical recurrence (BR). OCT4A$^{+}$ OCT4B$^{−}$ patients had the shortest BCRFS, and positivity for OCT4B expression was an independent prognostic factor for BCRFS in the multivariate analysis. We conclude that the expression of OCT4B is a strong marker of good prognosis, and its presence is associated with a decreased likelihood of BR. Thus, OCT4B might represent a powerful clinical prognostic biomarker for PCa patients.
Starting Page	2665
Ending Page	2673
Page Count	9
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	34
Issue Number	5
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-05-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Immunohistochemistry OCT4 OCT4A OCT4B Prostate cancer Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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