NDLI: Liver cancer-related gene CYP2E1 expression in HBV transgenic mice with acute liver injury

Content Provider	Springer Nature Link
Author	Zhang, Chun Wei, Qin Jiang, Tao Shou, Xi Li, Zhi Qiang Wen, Hao
Copyright Year	2013
Abstract	The objective of this research was to study the CYP2E1 gene expression in carbon tetrachloride (CCl$_{4}$)-induced acute liver injury in hepatitis B virus (HBV) transgenic mice. Twenty-four HBV (−) and 24 HBV (+) transgenic mice aged 8 to 10 weeks were selected for the present study. Intraperitoneal injection of 1.0 μL/g of CCl$_{4}$ (1:4 dissolved in olive oil) to mice was performed to induce acute liver injury model. Eight normal clean-grade C57BL/6 mice were taken as the control group. The control group received saline intraperitoneally. The mice in each group were killed 3, 6, 12, 24, 48, and 72 h after injection. The liver tissue samples of mice were collected. The liver histological changes at different time points in each group were observed under light microscope. The quantitative PCR methods were utilized to measure the relative mRNA levels of CYP2E1 gene in liver tissues. Immunohistochemistry and Western blot techniques were used to observe tissue expression levels of CYP2E1 in each group. Compared with that of the control group, mRNA and protein expression levels of CYP2E1 significantly increased both in the HBV (−) group and in the HBV (+) group after the CCl$_{4}$ induced the acute liver injury, and it reached the peak at 72 h after the CCl$_{4}$ injection. Compared with the HBV (−) group, the mice in the HBV (+) group had severe liver damage and significantly increased CYP2E1 gene and protein expression levels. In the CCl$_{4}$-induced acute liver injury of HBV transgenic mice, the CYP2E1 gene expression significantly increased. The results provided evidence for the HBV-induced liver damage and liver cancer pathogenesis.
Starting Page	3671
Ending Page	3677
Page Count	7
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	35
Issue Number	4
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-12-08
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Gene expression Liver Mice CYP2E1 Acute liver injury Carbon tetrachloride Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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