NDLI: Reduced mRNA expression levels of MBD2 and MBD3 in gastric carcinogenesis

Content Provider	Springer Nature Link
Author	Pontes, Thaís Brilhante Chen, Elizabeth Suchi Gigek, Carolina Oliveira Calcagno, Danielle Queiroz Wisnieski, Fernanda Leal, Mariana Ferreira Demachki, Samia Assumpção, Paulo Pimentel Artigiani, Ricardo Lourenço, Laércio Gomes Burbano, Rommel Rodriguez Arruda Cardoso Smith, Marília
Copyright Year	2013
Abstract	Aberrant methylation has been reported in several neoplasias, including gastric cancer. The methyl-CpG-binding domain (MBD) family proteins have been implicated in the chromatin remodeling process, leading to the modulation of gene expression. To evaluate the role of MBD2 and MBD3 in gastric carcinogenesis and the possible association with clinicopathological characteristics, we assessed the mRNA levels and promoter methylation patterns in gastric tissues. In this study, MBD2 and MBD3 mRNA levels were determined by RT-qPCR in 28 neoplastic and adjacent nonneoplastic and 27 gastritis and non-gastritis samples. The promoter methylation status was determined by bisulfite sequencing, and we found reduced MBD2 and MBD3 levels in the neoplastic samples compared with the other groups. Moreover, a strong correlation between the MBD2 and MBD3 expression levels was observed in each set of paired samples. Our data also showed that the neoplastic tissues exhibited higher MBD2 promoter methylation than the other groups. Interestingly, the non-gastritis group was the only one with positive methylation in the MBD3 promoter region. Furthermore, a weak correlation between gene expression and methylation was observed. Therefore, our data suggest that DNA methylation plays a minor role in the regulation of MBD2 and MBD3 expression, and the presence of methylation at CpGs that interact with transcription factor complexes might also be involved in the modulation of these genes. Moreover, reduced mRNA expression of MBD2 and MBD3 is implicated in gastric carcinogenesis, and thus, further investigations about these genes should be conducted for a better understanding of the role of abnormal methylation involved in this neoplasia.
Starting Page	3447
Ending Page	3453
Page Count	7
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	35
Issue Number	4
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-12-13
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Methyl-binding domain proteins Gastric cancer DNA methylation Gene expression Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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