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| Content Provider | PubMed Central |
|---|---|
| Author | Choi, Seon-ae Park, Chul Soon Kwon, Oh Seok Giong, Hoi-khoanh Lee, Jeong-soo Hwan, Ha Tai Lee, Chang-soo |
| Copyright Year | 2016 |
| Abstract | Hydrogen sulfide (H2S) is an important biological messenger, but fewbiologically-compatible methods are available for its detection in aqueous solution.Herein, we report a highly water-soluble naphthalimide-based fluorescent probe(L 1 ), which is a highly versatile building unit thatabsorbs and emits at long wavelengths and is selective for hydrogen sulfide overcysteine, glutathione, and other reactive sulfur, nitrogen, and oxygen species inaqueous solution. We describe turn-on fluorescent probes based on azide groupreduction on the fluorogenic ‘naphthalene’ moiety tofluorescent amines and intracellular hydrogen sulfide detection without the use ofan organic solvent. L 1 and L 2 weresynthetically modified to functional groups with comparable solubility on theN-imide site, showing a marked change in turn-on fluorescent intensity in responseto hydrogen sulfide in both PBS buffer and living cells. The probes were readilyemployed to assess intracellular hydrogen sulfide level changes by imagingendogenous hydrogen sulfide signal in RAW264.7 cells incubated withL 1 and L 2 . Expanding theuse of L 1 to complex and heterogeneous biological settings,we successfully visualized hydrogen sulfide detection in the yolk, brain and spinalcord of living zebrafish embryos, thereby providing a powerful approach for liveimaging for investigating chemical signaling in complex multicellular systems. |
| Related Links | http://dx.doi.org/10.1038/srep26203 |
| Starting Page | 26203 |
| File Format | |
| ISSN | 20452322 |
| e-ISSN | 20452322 |
| Journal | Scientific Reports |
| Volume Number | 6 |
| Language | English |
| Publisher | Nature Publishing Group |
| Publisher Date | 2016-05-01 |
| Access Restriction | Open |
| Rights Holder | Nature Publishing Group |
| Subject Keyword | Science and technology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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