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| Content Provider | PubMed Central |
|---|---|
| Author | Zheng, Xuexing Wong, Gary Zhao, Yongkun Wang, Hualei He, Shihua Bi, Yuhai Chen, Weijin Jin, Hongli Gai, Weiwei Chu, Di Cao, Zengguo Wang, Chong Fan, Quanshui Chi, Hang Gao, Yuwei Wang, Tiecheng Feng, Na Yan, Feihu Huang, Geng Zheng, Ying Li, Nan Li, Yuetao Qian, Jun Zou, Yong Gary, Kobinger Gao, George Fu Qiu, Xiangguo Yang, Songtao Xia, Xianzhu |
| Copyright Year | 2016 |
| Abstract | Recent successes with monoclonal antibody cocktails ZMappTM and MIL77 against Ebola virus (EBOV) infections have reignited interest in antibody-based therapeutics. Since the production process for monoclonal antibodies can be prolonged and costly, alternative treatments should be investigated. We produced purified equine antisera from horses hyperimmunized with EBOV virus-like particles, and tested the post-exposure efficacy of the antisera in a mouse model of infection. BALB/c mice were given up to 2 mg of purified equine antisera per animal, at 30 minutes, 1 or 2 days post-infection (dpi), in which all animals survived. To decrease the possibility of serum sickness, the equine antisera was digested with pepsin to generate F(ab′)2 fragments, with in vitro neutralizing activity comparable to whole immunoglobulin. Full protection was achieved with when treatment was initiated at 1 dpi, but the suboptimal protection observed with the 30 minute and 2 dpi groups demonstrate that in addition to virus neutralization, other Fc-dependent antibody mechanisms may also contribute to survival. Guinea pigs given 20 mg of antisera or F(ab′)2 at or starting at 1 or 2 dpi were also fully protected from EBOV infection. These results justify future efficacy studies for purified equine products in NHPs. |
| Related Links | http://dx.doi.org/10.1038/srep24179 |
| Starting Page | 24179 |
| File Format | |
| ISSN | 20452322 |
| e-ISSN | 20452322 |
| Journal | Scientific Reports |
| Volume Number | 6 |
| Language | English |
| Publisher | Nature Publishing Group |
| Publisher Date | 2016-04-01 |
| Access Restriction | Open |
| Rights Holder | Nature Publishing Group |
| Subject Keyword | Science and technology Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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