NDLI: The Caenorhabditis elegans protein SAS-5 forms large oligomeric assemblies critical for centriole formation

Content Provider	PubMed Central
Author	Robinson, Carol V. Rogala, Kacper B. Dynes, Nicola J. Hatzopoulos, Georgios N. Yan, Jun Pong, Sheng Kai Deane, Charlotte M. Pierre, Gönczy Vakonakis, Ioannis
Editor	Hyman, Anthony A.
Copyright Year	2015
Abstract	Centrioles are microtubule-based organelles crucial for cell division, sensing and motility. In Caenorhabditis elegans, the onset of centriole formation requires notably the proteins SAS-5 and SAS-6, which have functional equivalents across eukaryotic evolution. Whereas the molecular architecture of SAS-6 and its role in initiating centriole formation are well understood, the mechanisms by which SAS-5 and its relatives function is unclear. Here, we combine biophysical and structural analysis to uncover the architecture of SAS-5 and examine its functional implications in vivo. Our work reveals that two distinct self-associating domains are necessary to form higher-order oligomers of SAS-5: a trimeric coiled coil and a novel globular dimeric Implico domain. Disruption of either domain leads to centriole duplication failure in worm embryos, indicating that large SAS-5 assemblies are necessary for function in vivo.
Related Links	http://dx.doi.org/10.7554/eLife.07410
Starting Page	7410
File Format	PDF
ISSN	2050084X
e-ISSN	2050084X
Journal	eLife
Volume Number	4
Language	English
Publisher	eLife Sciences Publications, Ltd
Publisher Date	2015-05-29
Access Restriction	Open
Rights Holder	eLife Sciences Publications, Ltd
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Neuroscience Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology

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The Caenorhabditis elegans protein SAS-5 forms large oligomeric assemblies critical for centriole formation

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The Caenorhabditis elegans protein SAS-5 forms large oligomeric assemblies critical for centriole formation

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The Caenorhabditis elegans protein SAS-5 forms large oligomeric assemblies critical for centriole formation