NDLI: Preliminary investigation on cell biocompatibility of hydrated bacterial cellulose

Content Provider	IEEE Xplore Digital Library
Author	Yuanyuan Jia Renbo Tan Yan Wang Qijiang Lin Yi Shi Yulai Zhou
Copyright Year	2010
Description	Author affiliation: Experimental Center of Bioengineering, Pharmaceutical School, Jilin University, Changchun, 130021, China (Renbo Tan; Yan Wang; Qijiang Lin; Yi Shi; Yulai Zhou) \|\| Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, 300457, China (Yuanyuan Jia)
Abstract	In order to explore the possibility of using bacterial cellulose (BC) as scaffold in tissue engineering, fibroblasts (FB) were cultured as seed cells on hydrated membrane of bacterial cellulose (BC). First, FBs were isolated by dispase-collagenase system from the dermis of the back skin of newborn (within 24 hours) rats and were adherently cultured in DMEM medium with newborn calf serum (10%) under 5% CO2 and 37°C at a density of 1.0×105 cell/ml. The growth curve of FBs was determined by MTT method. Second, the subcultured rat FBs were inoculated on hydrated membrane of BC as scaffold, at a density of $3.0×10^{5}$ cell/ml. At different time intervals, the growth of cells on scaffold was determined by morphological characterization, such as HE staining, optical microscope, and scanning electron microscope (SEM). From the third to the seventh day of culture, the rat FBs were in logarithmic growth phase and showed strong proliferation, which might be well suited to be seed cells cultured on BC scaffold. After the inoculation for 3 days, rat FBs entered into the scaffold, and integrated closely with BC, which supported proliferation of cells. Excellent biocompatibility was verified in our experiment. The results suggested the potential for this biomaterial as a scaffold for tissue engineering.
Starting Page	1710
Ending Page	1713
File Size	4013449
Page Count	4
File Format	PDF
ISBN	9781424464951
e-ISBN	9781424464982
DOI	10.1109/BMEI.2010.5639737
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-10-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Scanning electron microscopy rat fibroblast (FB) Microorganisms cell compatibility tissue engineering Tissue engineering Fibroblasts Biomembranes Delta modulation bacterial cellulose (BC)
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in