NDLI: Rapid quantitative image analysis of hCG by gold immunochromatographic assay and genetic fast FCM algorithm

Content Provider	IEEE Xplore Digital Library
Author	Nianyin Zeng Yurong Li Min Du
Copyright Year	2010
Description	Author affiliation: Fujian Key Laboratory of Medical Instrumentation & Pharmaceutical Technology, Fuzhou, China (Min Du) \|\| College of Electrical Engineering and Automation, Fuzhou University, China (Nianyin Zeng; Yurong Li)
Abstract	In this paper, a CCD-based imaging gold immunochromatographic assay system combining genetic fast FCM algorithm is developed for rapid quantitative detection of human chorionic gonadotropin(hCG). The image of gold immunochromatographic strip is taken by CCD sensor, and after using the genetic fast FCM algorithm to precisely extract the test and control lines of strips, the reflective integral optical density(IOD) is selected as the feature based on Lambert-beer law. The ratio (IODt/IODc) is directly proportional to the concentration of hCG in a sample by which the interference in the test and control lines can be cancelled out each other. We observe a good linearity(correlation of coefficient r = 0.98995) from the construction of standard curve throughout the entire measuring range 0–500mIU/ml, and the detection limit of the proposed method is enough sensitive to detect the hCG in the blood or urine of the pregnancy woman. The precision of the intra-assay expressed as coefficient of variation(CV) is below 12%. The system presented here takes less than 10min to perform from the sample treatment to the data analysis. Relative to scanning reflective optical system, our results suggest that a simple CCD-based imaging system can speed assay development, reduce errors, and improve accuracy.
Starting Page	1560
Ending Page	1564
File Size	524593
Page Count	5
File Format	PDF
ISBN	9781424464951
e-ISBN	9781424464982
DOI	10.1109/BMEI.2010.5639261
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-10-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Strips Gold Image segmentation genetic fast FCM algorithm human chorionic gonadotropin image segmentation Clustering algorithms Optical imaging Optical reflection gold immunochromatographic assay quantitative test Immune system
Content Type	Text
Resource Type	Article

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