NDLI: Immunoassay by hydride generation-atomic fluorescence spectrometer using arsenic absorbed Fe3O4@Au nanoparticles as label

Content Provider	IEEE Xplore Digital Library
Author	Hai-juan Jin Jian-Guo Hou Tian-hua Li Ning Gan
Copyright Year	2010
Description	Author affiliation: Faculty of Material Science and Chemical Engineering, Ningbo University, China (Hai-juan Jin; Jian-Guo Hou; Tian-hua Li; Ning Gan)
Abstract	A novel immunoassay method based on hydride generation-atomic fluoresce spectrometer (HG-AFS) with arsenic (As) absorbed Fe3O4@Au (Fe3O4@Au-As) nanoparticles as tags to the antibody for the rapid and early diagnosis of α-fetoprotein (AFP) in primary carcinoma of the liver is proposed. The new approach combined the use of Fe3O4@Au-As nanoparticles labeled antibody, and HG-AFS detection for sandwich-type immunoassay. Firstly, Fe3O4@Au-As nanoparticles were prepared. Then Fe3O4@Au-As composite is used to immoblize HRP-labeled anti-AFP as secondary antibody (bionanospheres). The bionanospheres were characterized by transmission electron microscopy (TEM), and ultraviolet visible spectroscopy (UV-Vis). Sandwich-type protocol was successfully introduced to develop a new high-efficiency HG-AFS immunoassay. An important advantage of HG-AFS is high sensitivity to the detection of As. And in the immune-complex the concentration of As is high and has a linear relationship with AFP antigen, which could enlarge the signals. The antigen concentration, thus, was determined by measuring the arsenic in the immune complex with HG-AFS, which is quite promising for the detection of low concentration AFP at early stage of primary carcinoma of the liver.
Starting Page	1507
Ending Page	1510
File Size	359020
Page Count	4
File Format	PDF
ISBN	9781424464951
e-ISBN	9781424464982
DOI	10.1109/BMEI.2010.5639410
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-10-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Nanoparticles Gold Spectroscopy Absorption nanoparticles Fluorescence HG-AFS Nanobioscience Immune system immunoassay
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in