NDLI: Derivation of oocyte-like cells from mouse embryonic stem cells

Content Provider	IEEE Xplore Digital Library
Author	Yue Hu Shaohui Pan Jing Wang Shanshan Zhang Long Wang Jinlian Hua
Copyright Year	2010
Description	Author affiliation: College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Key Lab for Reproductive, Physiology& Embryo Biotechnology of Agriculture Ministry of China, Shaanxi Key Lab for Agriculture Molecular, Biotechnology Centre, Northwest A&F University, Yangling, 712100 China (Yue Hu; Shaohui Pan; Jing Wang; Shanshan Zhang; Long Wang; Jinlian Hua)
Abstract	In vitro derivation of oocytes from mouse embryonic stem cells (ESCs) would benefit the study of oogenesis. Here, we induced mouse ESCs to differentiate into oocyte-like cells by culturing adherent embryoid bodies (EBs) in different medium, including retinoic acid (RA) and follicular fluid. The extent of differentiation was evaluated through morphology and the expression of certain specific germ cell markers such as Oct4, Vasa, Scp3, Zp3, Gdf9 and Figla. As a result, EBs developed into ovary-like structures that contained oocyte-like cells showing expression of Oct4, Vasa, Gdf9, Figla and meiotic specific gene Scp3 and oocyte marker-Zp3. This study shows that RA and follicular fluid are beneficial for the derivation of germ cells and oocyte-like cells from mouse ES cells. The derivation of oocyte-like cells by our method offers an approach for studying oogenesis and regulation mechanism of mouse ESCs differentiation.
Starting Page	1659
Ending Page	1663
File Size	832086
Page Count	5
File Format	PDF
ISBN	9781424464951
e-ISBN	9781424464982
DOI	10.1109/BMEI.2010.5639615
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-10-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	mouse Fluids oocyte-like cells Humans Surface morphology embryonic stem cells Stem cells Mice In vitro Suspensions retinoic acid (RA) follicular fluid
Content Type	Text
Resource Type	Article

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