NDLI: Cellular tensegrity modeling with Atomic Force Microscopy (AFM) experimentation

Content Provider	IEEE Xplore Digital Library
Author	Ruiguo Yang Ning Xi Fung, C.K.M. Seiffert-Sinha, K. King Wai Chiu Lai Bo Song Sinha, A.A.
Copyright Year	2010
Description	Author affiliation: Department of Electrical and computer Engineering (Ruiguo Yang; Ning Xi; Fung, C.K.M.; King Wai Chiu Lai; Bo Song) \|\| Division of Dermatology and Cutaneous Sciences, Center for Investigative Dermatology, Michigan State University, East Lansing, MI USA (Seiffert-Sinha, K.; Sinha, A.A.)
Abstract	Cell tensegrity model has been widely accepted as a qualitative and recently quantitative method to describe the cellular mechanics. It is based on the fact the cell body is an inhomogeneous cytoskeleton based structure bounded by a soft membrane. Cell establishes force balance under certain structural arrangement through its focal adhesions and intercellular adhesions, under which the prestress is the main factor in determining the cell mechanical property as a whole. Here we demonstrate that intercellular adhesion is one of the main mechanisms employed by epithelial cells to achieve balance. We use keratinocytes as the model system to study this cellular behavior. It is found that loss of intercellular adhesion by desmosome disruption will cause the structural rearrangement of the cytoskeleton and subsequently lower the prestress in the whole cytoskeletal structure, thus change the mechanical property, in this case decrease of stiffness. The loss of intercellular adhesion causes was achieved by three different mechanisms either biochemical or biomechan-ical. Biochemically, antibody binding and calcium depletion would cause the disruption or non-formation of desmosome, which leads to loss of welding point of intermediate filaments. Biomechanically, intermediate filaments bundles were cut off by Atomic Force Microscopy (AFM) based nanodissection. All these mechanisms verified that the cell stiffness drop after intercellular adhesion loss. Further, simulation results by a 6-strut tensegrity structure with or without intermediate filaments confirmed the experimental findings, where structures with intermediate filaments are stiffer overall. This study would significantly enhance our understanding of the cell cytoskeleton mechanics.
Starting Page	65
Ending Page	70
File Size	282458
Page Count	6
File Format	PDF
ISBN	9781424488964
e-ISBN	9781424488971
DOI	10.1109/NMDC.2010.5652145
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-10-12
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Proteins Adhesives Calcium Force Nanobioscience Junctions
Content Type	Text
Resource Type	Article

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