NDLI: Comparing nanodroplets and microbubbles for enhancing ultrasound-mediated gene transfection

Content Provider	IEEE Xplore Digital Library
Author	Paproski, R.J. Zemp, R.J.
Copyright Year	2013
Description	Author affiliation: Dept. of Electr. & Comput. Eng., Univ. of Alberta, Edmonton, AB, Canada (Paproski, R.J.; Zemp, R.J.)
Abstract	Ultrasound is capable of transfecting cells with DNA through the process of sonoporation. Cell transfection by ultrasound alone is a relatively inefficient process but sonicating cells in the presence of microbubbles can significantly increase transfection efficiency. Unfortunately, microbubbles are relatively large (~3 μm diameter) and cannot leave the vasculature, preventing their direct interaction with cancer cells in solid tumors. Perfluorobutane microbubbles can be condensed into submicron nanodroplets that may be able to extravasate out of the tumor vasculature, potentially allowing these nanodroplets to accumulate in tumors. The current study compares microbubbles and nanodroplets for their abilities to enhance ultrasound-mediated transfection of cultured HEK293 cells. The nanodroplets in the current study were almost 6-fold smaller than their corresponding microbubbles and could be phase changed back into microbubbles with 400 cycles of 1.7 mechanical index ultrasound. Given enough cycles of ultrasound, low concentrations of nanodroplets (<; 1% v/v) were just as effective as microbubbles for enhancing ultrasound-mediated transfection of pEGFP-C1 DNA into HEK293 cells and high concentrations of nanodroplets (> 2 % v/v) were 2- to 3-fold more effective than microbubbles for transfecting HEK293 cells. The current study supports the use of nanodroplets to enhance ultrasound-mediated transfection due to their equivalent or greater transfection enhancing abilities as well as their smaller size which may allow nanodroplets to accumulate in tumors.
Sponsorship	IEEE Ultrason., Ferroelectr., Freq. Control Society
Starting Page	65
Ending Page	67
File Size	79857
Page Count	3
File Format	PDF
ISSN	19485719
e-ISBN	9781467356862
DOI	10.1109/ULTSYM.2013.0017
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2013-07-21
Publisher Place	Czech Republic
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Ultrasonic imaging DNA Tumors Cells (biology) Cancer Lipidomics Microscopy transfection Nanodroplets microbubbles perfluorobutane ultrasound
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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