NDLI: Difference proportional cell contact platform for 3D hepatocyte culture

Content Provider	IEEE Xplore Digital Library
Author	Yu-Shih Chen Zong-Keng Kuo Ling-Yi Ke Chiou-Wen Lin Hsiang-Wen Tseng Cheng-Hsien Liu
Copyright Year	2012
Description	Author affiliation: Biomedical Engineering Research Lab, Industrial Technology Research Institute, Hsinchu, Taiwan, R.O.C (Zong-Keng Kuo; Hsiang-Wen Tseng) \|\| Institute of NanoEngineering and MicroSystems, National Tsing Hua University, Hsinchu, Taiwan, R.O.C (Yu-Shih Chen; Cheng-Hsien Liu) \|\| Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan, R.O.C (Ling-Yi Ke; Chiou-Wen Lin)
Abstract	This paper reports a difference proportional cell contact platform for three dimension hepatocyte culture. We designed the cell wires about the width of 25 micrometer by dielectrophoresis for difference contact area. The platform was consisted of two dimensional cell contact chambers and three dimensional liver tissue chambers by dielectrophoresis pattern method and microfluidic channel design. For cell contact chambers, we used dielectrophoresis pattern method to make difference contact area for difference proportional cell communication. For liver tissue chambers, we used the UV-initiated solution of the poly (ethylene glycol)-diacrylate (PEG-DA) had been exposed to form microwells to pattern three dimension lobule-mimetic structure liver tissue in vitro. HepG2 cells were loading with three dimensional PEG-DA-dased micro-structures for artificial lobule pattern. Finally, we showed liver functional albumin testing. The albumin secretion of liver pattern chambers with HMEC-1 and C2C12 co-culture communication was 34% higher than the albumin secretion of liver chip without cells communication.
Starting Page	191
Ending Page	194
File Size	1277007
Page Count	4
File Format	PDF
ISBN	9781467311229
e-ISBN	9781467311243
DOI	10.1109/NEMS.2012.6196754
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2012-03-05
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Photonics Liver Dielectrophoresis Loading Titanium Barium dielectrophoresis Microfluidics Circulatory System cell pattern
Content Type	Text
Resource Type	Article

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