NDLI: In-Vitro Rapid Centimeter-Scale Reconstruction of Lobule-Mimetic Liver Tissue Employing Dielectrophoresis-Based Cell Patterning

Content Provider	IEEE Xplore Digital Library
Author	Chen-Ta Ho Ruei-Zeng Lin Hwan-You Chang Cheng-Hsien Liu
Copyright Year	2007
Description	Author affiliation: Nat. Tsing-Hua Univ., Hsinchu (Chen-Ta Ho)
Abstract	A lobule-mimetic cell-patterning technique for on-chip reconstructing centimeter-scale liver tissue of heterogeneous hepatic and endothelial cells via the enhanced field-induced dielectrophoresis (DEP) trap is demonstrated and reported. By mimicking the basic morphology of the liver tissue, the classic hepatic lobule, the lobule-mimetic-stellate-electrodes array is designed for cell patterning via DEP operation. Through the vertical positive DEP manipulation, well-defined and enhanced spatial electric field gradients are created for in-parallel manipulating of massive individual cells. Experiment results show that both hepatic and endothelial cells are orderly guided, snared, and aligned along the field-induced orientation to from the lobule-mimetic pattern containing of multiple radial hepatic cell-strings interlaced with radial endothelial cell-strings. About 95% cell viability of hepatic and endothelial cells is also observed after cell-patterning demonstration via the fluorescent assay technique.
Starting Page	351
Ending Page	354
File Size	882741
Page Count	4
File Format	PDF
ISBN	1424408415
DOI	10.1109/SENSOR.2007.4300140
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2007-06-10
Publisher Place	France
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	In vitro Liver Dielectrophoresis Electrodes Morphology Tissue engineering Electronic mail Regeneration engineering Microfluidics Energy resolution dielectrophoresis liver cell patterning
Content Type	Text
Resource Type	Article

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