NDLI: In-vitro transgenic mice liver tissue culture via hydrodynamic flow perfusion bioreactor

Content Provider	IEEE Xplore Digital Library
Author	Chen-Wei Wu Sivashankar, S. Valagerahally Puttaswamy, S. Hui-Ling Lin Kuo-Wei Chang Chau-Ting Yeh Cheng-Hsien Liu
Copyright Year	2012
Description	Author affiliation: Liver Research Unit, Chang Gung Memorial Hospital, Linkou, Taiwan, R.O.C (Hui-Ling Lin) \|\| Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan, R.O.C (Chen-Wei Wu; Sivashankar, S.; Valagerahally Puttaswamy, S.; Kuo-Wei Chang; Cheng-Hsien Liu)
Abstract	We report a Poly methyl methacrylate (PMMA) bioreactor in which hepatitis B virus (HBV) infected transgenic mice liver tissue has been cultured for long term with the continuous flow of culture medium, surrounded by mesothelial cells to provide 3-dimensional culturing conditions with adequate nutrient exchange. PDMS membrane was fabricated and a layer of mesothelial cells were cultured on it. Further this membrane was deformed and positioned on the microchannel by suction through the holes at the bottom of the channel. Liver tissue of transgenic mice was introduced on PDMS membrane. The experimental results proved that the proposed bioreactor portrays the in-vivo conditions better, with substantial improvement of liver-specific functions such as sufficient antigen expression, better structural integrity when compared to conventional static culture method. The in-vitro culture period of sliced liver tissue in our bioreactor was extended for 9 days to facilitate drug screening applications.
Starting Page	133
Ending Page	136
File Size	1046807
Page Count	4
File Format	PDF
ISBN	9781467311229
e-ISBN	9781467311243
DOI	10.1109/NEMS.2012.6196740
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2012-03-05
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Biomembranes Animals Nanoelectromechanical systems Biological system modeling Transgenic Mice Bioreactor Hydrodynamic Flow Tissue Culture
Content Type	Text
Resource Type	Article

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