NDLI: Control of biological clock activity capsulated by lipid-mono-layer

Content Provider	IEEE Xplore Digital Library
Author	Kojima, M. Nakajima, M. Takiguchi, K. Homma, M. Kondo, T. Fukuda, T.
Copyright Year	2012
Description	Author affiliation: Department of Micro-Nano Systems Engineering, and with the Center for Micro-Nano Mechatronics Nagoya University, Furo-cho, Chikusa-ku, 464-8603 JAPAN (Fukuda, T.) \|\| Division of biological science, Nagoya University, Furo-cho, Chikusa-ku, 464-8602 JAPAN (Takiguchi, K.; Homma, M.; Kondo, T.) \|\| Department of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, 464-8603 JAPAN (Kojima, M.) \|\| Center for Micro-Nano Mechatronics, Nagoya University, Furo-cho, Chikusa-ku, 464-8603 JAPAN (Nakajima, M.)
Abstract	In this paper, we try to establish new technique that the components of the biological clock are reconstituted into the liposome. In other words, we try to produce a nano size clock, capsulated into the liposome, made by protein molecules. The circadian clock is a basic cellular system found in almost all organisms. This clock generates self-sustained oscillations under constant conditions with a ≈ 24-hour (circadian) period. In cyanobacteria, circadian clock could be reconstituted in vitro only by mixing the three clock proteins, KaiA, KaiB, KaiC, with adenosine triphosphate (ATP). So we reconstitute these proteins and adenosine triphosphate (ATP) into phospholipid-coated microdroplet and confirmed the clock function. The clocks in phospholipids-coated microdroplet indicate long period more than 24 hours. In this case, period length became 35 hour, self-sustaining oscillation was reaming with little dumping. To reveal why the time cycle became long period, we observed localization of Kai proteins in phospholipid-coated microdroplets by using fluorescents labeled Kai proteins under fluorescent (confocal) microscopy. From the observation of localization of Kai proteins, we found KaiB protein was distributed equivalently, on the other hand, KaiC protein was located near membrane of phospholipid-coated microdroplet. These results indicate that different localization between Kai proteins cause long period oscillation and we could control period length depend on calculation data from localization.
Starting Page	2196
Ending Page	2201
File Size	1097499
Page Count	6
File Format	PDF
ISBN	9781467314039
ISSN	10504729
e-ISBN	9781467314053
e-ISBN	9781467314046
DOI	10.1109/ICRA.2012.6225353
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2012-05-14
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Proteins Fluorescence Clocks Chronobiology Microscopy Biomembranes Oscillators
Content Type	Text
Resource Type	Article
Subject	Artificial Intelligence Control and Systems Engineering Electrical and Electronic Engineering Software

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