NDLI: Control of cell detachment on poly(N-isopropylacrylamide) grafted glass surfaces

Content Provider	IEEE Xplore Digital Library
Author	Fukumori, K. Akiyama, Y. Kikuchi, A. Yamato, M. Sakai, K. Okano, T.
Copyright Year	2006
Description	Author affiliation: Dep. Appl. Chem., Waseda Univ., Tokyo (Fukumori, K.)
Abstract	IPAAm grafted glass coverslips was prepared (PIPAAm-CS) using electron beam and coverslips modified with MPTMS. XPS analysis of the PIPAAm-CS surfaces revealed that PIPAAm was successfully grafted onto the coverslips. Initial monomer concentration significantly influenced thickness and amount of the grafted PIPAAm. Surface wettability of PIPAAm-CSs increased with an increase in thickness and amount of the grafted PIPAAm. PIPAAm-CS with the grafted PIPAAm = 0.84 mug/cm2 (0.84PIPAAm-CS) showed cell attachment and detachment properties in response to temperature, while PIPAAm-CS having the grafted PIPAAm more than 1.28 $mu/cm^{2}$ exhibited cell repellent surfaces. Wettability of PIPAAm-CSs supports this cell adhesion behavior. AFM measurements of the PIPAAm grafted coverslips, which are subjected to locally limited UV laser ablation, demonstrate that the grafted PIPAAm is ultra thin layer. Thickness of 0.84PIPAAm-CS and 1.28PIPAAm-CS are 3.6 nm and 8.8 nm, respectively. We further discuss thickness-dependent cell adhesion properties in terms of different molecular motion of the grafted PIPAAm chains between PIPAAm-CSs with thinner and thicker PIPAAm layer, referring our previous report
Starting Page	1
Ending Page	5
File Size	4775403
Page Count	5
File Format	PDF
ISBN	1424407176
DOI	10.1109/MHS.2006.320284
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-11-05
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Glass Electron beams Adhesives Chemical industry Temperature Methanol Bovine Surface treatment Cascading style sheets Biomedical measurements
Content Type	Text
Resource Type	Article

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