NDLI: Characterization of the cellular biomechanlcal responses caused on microprocessed substrates: effect of micropatterned cell adhesiveness and microelasticity gradient

Content Provider	IEEE Xplore Digital Library
Author	Kidoaki, S. Matsuda, T.
Copyright Year	2006
Description	Author affiliation: Div. of Biomolecular Chem., Kyushu Univ., Fukuoka (Kidoaki, S.)
Abstract	To investigate the shape-dependent cell mechanics and ECM-dependent control of motility, cell elasticity, structural features of the actin cytoskeleton (CSK) and motility responses were characterized on well-designed microprocessed substrates with micropatterned cell adhesive regions and microelastic gradient. Cell elasticity and actin CSK features of shape-engineered fibroblasts and vascular endothelial cells (round and spindle-shaped) cultured on photolithographically microprocessed surfaces were characterized employing the cellular microindentation test and fluorescence observation of actin CSK by the combination of atomic force microscopy (AFM) and fluorescence microscopy (FM). Condition of directional cell movement towards stiffer region, so called mechanotaxis, was studied on the photolithographically-microprocessed microelastic gradient gel (MEG gel). The interrelationships between cell elasticity, the structural features of actin CSK and engineered cell shape were analyzed as compared with control cells cultured on non-processed surfaces (termed naturally extended cells). The results showed that the elasticity of regionally restricted adhesion-surface-induced shape-engineered cells, particularly highly elongated cells, is predominantly affected by cell shape rather than by the structural features of SFs. As for the mechanotaxis behaviors induced on MEG gel, design of both elasticity jump and discreteness in the elasticity boundary was found to be essential to cause cellular directional movement
Starting Page	1
Ending Page	5
File Size	6082788
Page Count	5
File Format	PDF
ISBN	1424407176
DOI	10.1109/MHS.2006.320327
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-11-05
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Shape control Cells (biology) Elasticity Adhesives Atomic force microscopy Chemical technology Electrochemical machining Fluorescence Extracellular Materials science and technology
Content Type	Text
Resource Type	Article

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