NDLI: Defatting heptocytes under flow

Content Provider	IEEE Xplore Digital Library
Author	Yarmush, J. Yarmush, G. Nativ, N. Berthiaume, F.
Copyright Year	2014
Description	Author affiliation: Dept. of Biomed. Eng., State Univ. of New Jersey, Piscataway, NJ, USA (Yarmush, J.; Yarmush, G.; Nativ, N.; Berthiaume, F.)
Abstract	There is a critical shortage of transplantable livers in both the US and the world. One method to increase the donor pool is to develop methodology to recondition extended criteria donor grafts, a large portion of which are moderate to severe macrosteatotic livers. Transplantation of these livers often leads to primary nonfunction caused by an increased susceptibility to the effects of ischemia reperfusion injury that result from the harvesting, transportation, and transplantation of the liver. Our lab has developed a novel cocktail of defatting reagents that can, over a period of two days, render hepatocytes lean and with good cell viability and function. Despite this accomplishment, in order to be feasibly performed in a clinical setting, the defatting process must be completed in a matter of hours. The current project focuses on understanding the differences between defatting in a static versus flow environment and then using this information to develop the ideal parameters for defatting whole organs. Our hypothesis in the current work is that using flow and the appropriate defatting agents, steatotic hepatocytes can be defatted in a clinically relevant time of hours, without decreasing cell viability or function. In order to test this hypothesis, a perfusion reactor was constructed which holds microscope slides that can be seeded with fatty hepatocytes. Perfusion of defatting media was performed at different flow rates over a six hour period. Results showed that within a six hour period, significant defatting (as compared to static cultures) was observed at both 2.0 and 4.0 ml/min as indicated by oil red O staining. Current experiments are focused on further evaluation of hepatocyte viability and function (e.g. albumin, urea, and cytochrome P450 function). These types of parametric experiments will form the basis for future perfusions with whole organs from obese rats.
Starting Page	1
Ending Page	2
File Size	4109005
Page Count	2
File Format	PDF
ISBN	9781479937288
DOI	10.1109/NEBEC.2014.6972985
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2014-04-25
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Liver Lipidomics Injuries Microscopy Standards Inductors Rats liver function Macrosteatosis hepatocytes flow device defatting
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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