NDLI: Fluorescence quantification of nanoparticle diffusion from smart brachytherapy spacers in vivo

Content Provider	IEEE Xplore Digital Library
Author	Markovic, S. Kumar, R. Belz, J. Sridhar, S. Niedre, M.
Copyright Year	2014
Description	Author affiliation: Dept. of Phys., Northeastern Univ., Boston, MA, USA (Kumar, R.; Belz, J.; Sridhar, S.) \|\| Dept. of Electr. & Comput. Eng., Northeastern Univ., Boston, MA, USA (Markovic, S.; Niedre, M.)
Abstract	New implantable smart nanoparticle (NP) coated brachytherapy spacers have recently been developed which allows controlled, long-term release of chemotherapeutic drugs into tumors. However, kinetics of NP (drug) diffusion over time is still poorly understood, and new methods for controlling and optimizing this release are needed. In this work, we developed and validated a novel broad-field transmission fluorescence imaging system to observe NP diffusion in bulk biological tissue. This allowed us to systematically quantify diffusion both in phantoms in vitro and in mice in vivo. We performed in vitro studies with free dye and NPs of different sizes (30 nm and 200 nm) in agar gel phantoms and analyzed the diffusion over time. It was found that the free dye diffusion coefficient was orders of magnitude larger than both NP types verifying that diffusion could be controlled based on particle size. We also performed preliminary studies in mice, where functionalized brachytherapy spacers were implanted into the hind flanks of nude mice. Continuous diffusion of free dye and NPs was observed, with maximal diffusion areas observed on day 4 and day 6 for 30 nm and 200 nm NPs, respectively. Our fluorescence imaging system was capable of robustly quantifying this size-dependent diffusion. In the future, we will use it to optimize and control drug delivery from smart nanoparticles over time.
Starting Page	1
Ending Page	2
File Size	4051443
Page Count	2
File Format	PDF
ISBN	9781479937288
DOI	10.1109/NEBEC.2014.6972866
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2014-04-25
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fluorescence In vivo Phantoms Imaging phantoms Mice Brachytherapy diffusion near infrared transmission nanoparticles
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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