NDLI: Insulator based dielectrophoresis to predict electrocompetency during electroporation

Content Provider	IEEE Xplore Digital Library
Author	Zhifei Ge Buie, C.
Copyright Year	2014
Description	Author affiliation: Dept. of Mech. Eng., Massachusetts Inst. of Technol., Cambridge, MA, USA (Zhifei Ge; Buie, C.)
Abstract	Limitations on engineering Escherichia coli to accept foreign DNAs require us to build more chasses for synthetic biology. Electroporation is one of most widely used process to introduce foreign DNA into cells. However, electroporation protocols exist for a limited number of species and the existing method to develop electroporation protocols require cells to go through the entire electroporation process, which is insufficient, especially for slow-growth species. To fast develop protocols, we are developing insulator-based dielectrophoresis to for optimization of electroporation protocols with the hypothesized correlation between polarizability and electrocompetency. The dielectrophoresis is used to quantify polarizability of cells under electric field. The hypothesis is made based on the fact that both dielectrophoresis and electroporation is a result of charge redistribution under electric potential. We developed methods to validate the correlation and our initial experimental results show that higher polarizability leads to higher electrocompetency for E. coli.
Starting Page	1
Ending Page	1
File Size	2409180
Page Count	1
File Format	PDF
ISBN	9781479937288
DOI	10.1109/NEBEC.2014.6972793
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2014-04-25
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Dielectrophoresis Protocols Correlation Electric fields Microorganisms Force Synthetic biology electrocompetency electroporation dielectropohoresis polarizability
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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