NDLI: Micro/nanorough titanium-aluminum-vanadium alloy surfaces trigger alternate osteoblast integrin expression profile

Content Provider	IEEE Xplore Digital Library
Author	Gittens, R.A. Sandhage, K.H. Olivares-Navarrete, R. Hyzy, S.L. Schwartz, Z. Boyan, B.D.
Copyright Year	2014
Description	Author affiliation: Dept. of Biomed. Eng., Virginia Commonwealth Univ. (VCU) Richmond, Richmond, VA, USA (Olivares-Navarrete, R.; Hyzy, S.L.; Schwartz, Z.; Boyan, B.D.) \|\| Sch. of Mater. Sci. & Eng., Georgia Inst. of Technol., Atlanta, GA, USA (Sandhage, K.H.) \|\| Center for Biodiversity & Drug Discovery, INDICASAT-AIP, Panama City, Panama (Gittens, R.A.)
Abstract	Recent studies of new surface modifications that superimpose well-defined nanostructures on microrough implants mimicking the hierarchical complexity of native bone, report synergistically enhanced osteoblast maturation and local factor production at the protein level compared to smooth, nanorough and microrough surfaces. Whether these micro/nanorough surfaces enhance the osteogenic response by triggering similar patterns of integrin receptors and their associated signaling pathways as with well-established microrough surfaces is not well understood. Human osteoblasts (hOBs) were cultured until confluent for gene expression studies on tissue culture polystyrene (TCPS) or on titanium alloy (Ti6Al4V) disks with different surface topography: smooth, nanorough, microrough and micro/nanorough surfaces. mRNA expression levels of osteogenesis-related markers such as osteocalcin (BGLAP) and bone sialoprotein (BSP), bone morphogenetic protein 2 (BMP2), BMP4, noggin (NOG) and gremlin 1 (GREM1) were all higher on microrough and micro/nanorough surfaces, with few differences between them, compared to smooth and nanorough groups. Interestingly, expression of integrins α1 and α2, which interact primarily with collagens and laminin and have been commonly associated with osteoblast differentiation on microrough Ti and Ti6Al4V, were expressed at lower levels on micro/nanorough surfaces compared to microrough ones. Conversely, the αv subunit, which binds ligands such as vitronectin, osteopontin, and bone sialoprotein among others, had higher expression on micro/nanorough surfaces concomitantly with regulation of the β3 mRNA levels on nanomodified surfaces. These results suggest that the maturation of osteoblasts on micro/nanorough surfaces may be occurring through different integrin engagement than those established for microrough-only surfaces.
Starting Page	1
Ending Page	2
File Size	386506
Page Count	2
File Format	PDF
ISBN	9781479937288
DOI	10.1109/NEBEC.2014.6972798
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2014-04-25
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Surface topography Gene expression Surface morphology Bones Surface treatment Rough surfaces Surface roughness mRNA implants osseointegration bone nanostructures surface properties gene expression
Content Type	Text
Resource Type	Article

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